豚鼠脑亚细胞组分中游离氨基酸的分布
The distribution of free amino acids in subcellular fractions of guinea-pig brain.
作者信息
Mangan J L, Whittaker V P
出版信息
Biochem J. 1966 Jan;98(1):128-37. doi: 10.1042/bj0980128.
Abstract
- The free amino acids of homogenates of guinea-pig brain in 0.32m-sucrose and of subcellular fractions derived therefrom have been estimated by the method of Moore & Stein. 2. Seven amino acids together accounted for over 80% of the free amino compounds; these are, in decreasing order of abundance: glutamate, aspartate, gamma-aminobutyrate, glycine, serine, alanine and threonine. In addition, there are appreciable quantities of amide (presumably glutamine). 3. Control experiments showed that the pattern of free amino acid occurrence in sucrose homogenates was similar to that of brains of animals killed by freezing in liquid nitrogen and extracted immediately without thawing. 4. The subcellular distribution of the amino acids resembled that of soluble cytoplasmic markers; there was no specific localization in a fraction rich in isolated presynaptic nerve terminals of amino acids capable of exciting or depressing central neurones. 5. The significance of the results is discussed in relation to the possible role of centrally active amino acids as transmitters.
摘要
- 采用摩尔和斯坦因的方法，对豚鼠脑在0.32m蔗糖溶液中的匀浆以及由此得到的亚细胞组分中的游离氨基酸进行了测定。2. 七种氨基酸占游离氨基化合物总量的80%以上；按含量递减顺序依次为：谷氨酸、天冬氨酸、γ-氨基丁酸、甘氨酸、丝氨酸、丙氨酸和苏氨酸。此外，还有相当数量的酰胺（可能是谷氨酰胺）。3. 对照实验表明，蔗糖匀浆中游离氨基酸的分布模式与液氮冷冻处死并立即在不解冻的情况下提取的动物脑相似。4. 氨基酸的亚细胞分布与可溶性细胞质标记物相似；在富含分离的突触前神经末梢的组分中，能够兴奋或抑制中枢神经元的氨基酸没有特异性定位。5. 结合具有中枢活性的氨基酸作为递质的可能作用，对结果的意义进行了讨论。
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