Shimizu N, Kondo I, Gamou S, Behzadian M A, Shimizu Y
Somat Cell Mol Genet. 1984 Jan;10(1):45-53. doi: 10.1007/BF01534472.
Human epidermoid carcinoma A431 cells, possessing an extraordinarily high number of epidermal growth factor (EGF) receptors (1), were found to be hypotetraploid in their chromosome constitution and to contain two copies of intact chromosome 7 and two types of the translocation chromosomes involving chromosome 7 (M4 and M14) as well as several other rearranged chromosomes. The A431 cells were fused with mouse A9 cells, which lack EGF receptors (2) and are deficient in hypoxanthine phosphoribosyltransferase (3), and the human-mouse cell hybrid (AA series) were selected in HAT/ouabain medium (3, 4). The expression of high EGF binding ability was correlated with the presence of human translocation chromosome M4. AA hybrid clones that contained intact human chromosome 7 but not the marker chromosome M4 expressed only ordinary levels of EGF receptors. The EGF receptors expressed in the AA hybrids were proven to be of human nature by immunoprecipitation of the receptors cross-linked with [125I]EGF. These observations and our previous gene assignment of the EGF receptor to human chromosome 7 (2, 5) suggest that the marker chromosome M4 may carry an alteration(s) in the gene(s) involved in EGF receptor biosynthesis.
人表皮样癌A431细胞具有数量异常多的表皮生长因子（EGF）受体（1），其染色体组成为亚四倍体，含有两条完整的7号染色体、两种涉及7号染色体的易位染色体（M4和M14）以及其他几条重排染色体。将A431细胞与缺乏EGF受体（2）且次黄嘌呤磷酸核糖转移酶缺陷（3）的小鼠A9细胞融合，并在HAT/哇巴因培养基（3,4）中筛选出人-鼠细胞杂种（AA系列）。高EGF结合能力的表达与人类易位染色体M4的存在相关。含有完整人类7号染色体但不含有标记染色体M4的AA杂种克隆仅表达正常水平的EGF受体。通过对与[125I]EGF交联的受体进行免疫沉淀，证明AA杂种中表达的EGF受体具有人类特性。这些观察结果以及我们之前将EGF受体基因定位于人类7号染色体的研究（2,5）表明，标记染色体M4可能携带参与EGF受体生物合成的基因的改变。
