Gill G N, Weber W, Thompson D M, Lin C, Evans R M, Rosenfeld M G, Gamou S, Shimizu N
Somat Cell Mol Genet. 1985 Jul;11(4):309-18. doi: 10.1007/BF01534689.
Synthesis of the epidermal growth factor (EGF) receptor has been analyzed in a series of variant A431 human epidermoid carcinoma cell clones reported to contain different amounts of EGF binding sites. The amount of EGF receptor protein, quantitated by immunoaffinity chromatography, and EGF receptor mRNA, quantitated by cDNA hybridization, were closely correlated to the extent of EGF receptor gene amplification. This correlation existed in variants selected for reduced EGF receptors and in revertants from those variants with increased EGF receptors. There was also a correlation between the frequency of translocation of chromosome 7, containing the EGF receptor gene, and EGF receptor protein. These results support gene amplification as the mechanism enhancing A431 cell EGF receptor protein and determining growth responses.
在一系列据报道含有不同数量表皮生长因子（EGF）结合位点的变异型A431人表皮样癌细胞克隆中，对EGF受体的合成进行了分析。通过免疫亲和层析定量的EGF受体蛋白量和通过cDNA杂交定量的EGF受体mRNA，与EGF受体基因扩增程度密切相关。这种相关性存在于为减少EGF受体而选择的变异体以及那些具有增加的EGF受体的变异体的回复体中。含有EGF受体基因的7号染色体易位频率与EGF受体蛋白之间也存在相关性。这些结果支持基因扩增是增强A431细胞EGF受体蛋白并决定生长反应的机制。
