Takai S, Higashihara M, Matumoto M
Arch Virol. 1984;80(1):59-67. doi: 10.1007/BF01315294.
We purified three populations of virus particles, F7, F9 and F17, with buoyant densities of 1.34, 1.33 and 1.29 g/ml, respectively, in CsCl equilibrium density gradients from cultures of chick embryo liver cells infected with the H-162 strain of the virus of egg drop syndrome 1976. F9 particles were infectious complete virions and most F17 particles were empty particles. F7 particles were less infectious, and had little capacity of hemagglutination (HA). HA titers were the same at 4 degrees and 37 degrees C and maximal between pH 6.4 and 8.4 and ionic strength from 0.14 to 0.54 M of NaCl. HA titer was inversely proportional to erythrocyte concentration. Potassium periodate destroyed markedly the infectivity of the virus and partially its HA activity at 37 degrees C. HA activity was stable at 56 degrees C or lower temperatures and destroyed at 80 degrees C. Trypsin, alpha-chymotrypsin, papain, ficin and neuraminidase had no effect on HA activity. Alpha-chymotrypsin destroyed the receptor for the virus on chicken erythrocytes, whereas trypsin and neuraminidase did not affect the receptor.
我们从感染了1976年蛋鸡减蛋综合征病毒H - 162株的鸡胚肝细胞培养物的氯化铯平衡密度梯度中，纯化出了三种病毒颗粒群体，分别为F7、F9和F17，其浮力密度分别为1.34、1.33和1.29 g/ml。F9颗粒是具有感染性的完整病毒粒子，而大多数F17颗粒是空粒子。F7颗粒的感染性较低，血凝（HA）能力也较弱。HA效价在4℃和37℃时相同，在pH值6.4至8.4以及氯化钠离子强度0.14至0.54 M之间达到最大值。HA效价与红细胞浓度成反比。高碘酸钾在37℃时显著破坏病毒的感染性并部分破坏其HA活性。HA活性在56℃或更低温度下稳定，在80℃时被破坏。胰蛋白酶、α - 糜蛋白酶、木瓜蛋白酶、无花果蛋白酶和神经氨酸酶对HA活性均无影响。α - 糜蛋白酶破坏鸡红细胞上病毒的受体，而胰蛋白酶和神经氨酸酶不影响该受体。
