Swain P, Kataria J M, Verma K C
Division of Avian Diseases, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh.
Res Vet Sci. 1993 Nov;55(3):396-7. doi: 10.1016/0034-5288(93)90118-y.
An isolate of egg drop syndrome-76 virus replicated best in primary chicken embryo liver cells and less well in duck embryo liver cells, duck embryo fibroblast cells and chicken embryo kidney cells. The cytopathic effect in chicken embryo liver cells was marked by the presence of round and refractile cells and detachment of cells from the glass surface. The intranuclear eosinophilic inclusion bodies were observed by 24 to 48 hours after infection. No virus multiplication was observed in primary quail embryo fibroblast cells, chicken embryo fibroblast cells or mammalian cells like Vero, BHK-21 and MDBK. Duck embryos supported the maximum growth of the virus, with allantoic fluid having the highest haemagglutinin titre, followed in order by chorioallantoic membrane, skin and internal organs. Chicken and quail embryos did not support the growth of the virus.
禽减蛋综合征-76病毒的一个分离株在原代鸡胚肝细胞中复制最佳，在鸭胚肝细胞、鸭胚成纤维细胞和鸡胚肾细胞中复制较差。鸡胚肝细胞中的细胞病变效应表现为出现圆形、折光性细胞以及细胞从玻璃表面脱落。感染后24至48小时可观察到核内嗜酸性包涵体。在原代鹌鹑胚成纤维细胞、鸡胚成纤维细胞或Vero、BHK-21和MDBK等哺乳动物细胞中未观察到病毒增殖。鸭胚支持该病毒的最大生长，尿囊液的血凝素效价最高，其次依次为绒毛尿囊膜、皮肤和内脏器官。鸡胚和鹌鹑胚不支持该病毒的生长。
