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来自感染单链DNA病毒的植物的负超螺旋DNA。

Negatively supercoiled DNA from plants infected with a single-stranded DNA virus.

作者信息

Sunter G, Coutts R H, Buck K W

出版信息

Biochem Biophys Res Commun. 1984 Feb 14;118(3):747-52. doi: 10.1016/0006-291x(84)91458-x.

Abstract

A method for isolating covalently closed circular double-stranded DNA from plants infected with the geminivirus, tomato golden mosaic virus, is described. Ethidium bromide titration showed this DNA to be negatively supercoiled with a superhelical density of -0.062. The presence of S1 nuclease-sensitive secondary structure in the supercoiled DNA was demonstrated by its conversion to the open circular and linear DNA forms on treatment with this enzyme.

摘要

描述了一种从感染双生病毒——番茄金色花叶病毒的植物中分离共价闭合环状双链DNA的方法。溴化乙锭滴定显示该DNA为负超螺旋,超螺旋密度为-0.062。超螺旋DNA中存在对S1核酸酶敏感的二级结构,这通过用该酶处理后其转变为开环和线性DNA形式得到证实。

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