Bartholomaeus W N, O'Donoghue H, Reed W D
Clin Exp Immunol. 1984 Mar;55(3):541-5.
Proposed mechanisms for the induction of autoantibodies to liver specific lipoprotein (LSP) assume that the autoantibody response is T-dependent. This hypothesis was tested in the athymic nude mouse. Athymic homozygote (nu/nu) nude mice and appropriate control mouse strains were immunized with rabbit or human LSP and infected with murine cytomegalovirus (MCMV) in an attempt to induce autoantibodies to LSP. Antibodies to LSP were measured by passive haemagglutination and by an enzyme linked immunosorbent assay. Nude mice did not produce antibodies to either foreign LSP species or autoantibodies to mouse LSP when immunized with either rabbit or human LSP. Control heterozygote (nu/+) mice and C57BL/6J mice produced antibody to foreign LSP and autoantibody to mouse LSP when immunized with xenogeneic LSP. Athymic nu/nu mice also failed to produce autoantibody to mouse LSP following infection with MCMV, in contrast to control nu/+ and C57BL/6J mice which produced LSP autoantibody after infection with MCMV. It is concluded that the autoantibody response to LSP is T-dependent.
针对肝特异性脂蛋白（LSP）自身抗体诱导的潜在机制假定自身抗体反应是T细胞依赖性的。该假设在无胸腺裸鼠中进行了验证。将无胸腺纯合子（nu/nu）裸鼠及相应的对照小鼠品系用兔或人LSP免疫，并感染鼠巨细胞病毒（MCMV），试图诱导针对LSP的自身抗体。通过被动血凝试验和酶联免疫吸附测定法检测针对LSP的抗体。当用兔或人LSP免疫时，裸鼠不会产生针对任何一种外源LSP的抗体，也不会产生针对小鼠LSP的自身抗体。对照杂合子（nu/+）小鼠和C57BL/6J小鼠在用异种LSP免疫时会产生针对外源LSP的抗体和针对小鼠LSP的自身抗体。与对照nu/+小鼠和C57BL/6J小鼠在感染MCMV后产生LSP自身抗体不同，无胸腺nu/nu小鼠在感染MCMV后也未能产生针对小鼠LSP的自身抗体。得出的结论是，针对LSP的自身抗体反应是T细胞依赖性的。
