Olver S D, Price P, Shellam G R
Department of Microbiology, University of Western Australia, Nedlands.
Clin Exp Immunol. 1994 Dec;98(3):375-81. doi: 10.1111/j.1365-2249.1994.tb05500.x.
Mice susceptible and resistant to murine cytomegalovirus (MCMV) were infected with this virus and livers were harvested after 2-231 days. Cryostat sections were stained to visualize cells bearing CD4, CD8 or Mac-1 antigens. Mac-1+ cells were prevalent in inflammatory foci after 2 days. These cells persisted in susceptible BALB/c and A/J mice, but disappeared from livers of resistant C57Bl/6 and CBA/CaH mice by day 28. T cell inflammation peaked on days 7-11. This declined by day 56 in C57Bl/6 and CBA/CaH mice, but persisted in BALB/c and A/J mice for at least 231 days. Persistent CD8+ cells were dispersed throughout the parenchyma. More CD8+ cells were observed 7-14 days after infection in the livers of bg/bg (natural killer (NK) cell-deficient) C57Bl/6 and CBA mice, and in C57Bl/6 mice depleted of NK1.1 cells by MoAb. Thus, mice of strains susceptible to MCMV exhibit hepatitis characterized by persistence of dispersed CD8+ cells. This phenomenon may be limited by NK cells in resistant strains.
将对鼠巨细胞病毒（MCMV）易感和有抗性的小鼠感染该病毒，并在2至231天后采集肝脏。用低温恒冷切片机切片并染色，以观察带有CD4、CD8或Mac-1抗原的细胞。2天后，Mac-1+细胞在炎症灶中普遍存在。这些细胞在易感的BALB/c和A/J小鼠中持续存在，但在第28天时从抗性C57Bl/6和CBA/CaH小鼠的肝脏中消失。T细胞炎症在第7至11天达到峰值。在C57Bl/6和CBA/CaH小鼠中，这种炎症在第56天时下降，但在BALB/c和A/J小鼠中至少持续231天。持续存在的CD8+细胞分散在整个实质组织中。在bg/bg（自然杀伤（NK）细胞缺陷型）C57Bl/6和CBA小鼠以及通过单克隆抗体耗尽NK1.1细胞的C57Bl/6小鼠的肝脏中，感染后7至14天观察到更多的CD8+细胞。因此，对MCMV易感品系的小鼠表现出以分散的CD8+细胞持续存在为特征的肝炎。这种现象在抗性品系中可能受到NK细胞的限制。
