Douglas J T, Rosenberg E Y, Nikaido H, Verstreate D R, Winter A J
Infect Immun. 1984 Apr;44(1):16-21. doi: 10.1128/iai.44.1.16-21.1984.
The outer membrane of Brucella species contains two major proteins, denoted as group 2 and group 3 proteins (Verstreate et al., Infect. Immun. 35:979-989, 1982). We reconstituted proteoliposomes from the purified proteins and egg phosphatidylcholine and showed that group 2 proteins, but not a group 3 protein, had the porin activity. The influx rates of sugars of various sizes into the proteoliposomes indicated that the porin channels had apparent diameters in a range comparable to that of Escherichia coli OmpF porin and that the channels were predominantly open. Among different Brucella species, there were small but detectable differences in the channel diameter, and it was possible to explain the differential sensitivity of several Brucella species to diagnostic dyes on the basis of these observed differences.
布鲁氏菌属的外膜含有两种主要蛋白质，分别称为2组和3组蛋白质（Verstreate等人，《感染与免疫》35:979 - 989，1982年）。我们用纯化的蛋白质和鸡蛋磷脂酰胆碱重构了蛋白脂质体，并表明2组蛋白质具有孔蛋白活性，而3组蛋白质没有。各种大小的糖类进入蛋白脂质体的流入速率表明，孔蛋白通道的表观直径范围与大肠杆菌OmpF孔蛋白相当，且通道主要是开放的。在不同的布鲁氏菌物种中，通道直径存在微小但可检测到的差异，基于这些观察到的差异有可能解释几种布鲁氏菌物种对诊断染料的不同敏感性。
