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牛外周血淋巴细胞的体外刺激:用于检测结核菌素超敏反应的平底和圆底微量滴定板的比较

In vitro stimulation of bovine peripheral blood lymphocytes: comparison of round- and flat-bottom microtiter plates for detection of tuberculin hypersensitivity.

作者信息

Sloane E D, Muscoplat C C, Kaneene J M, Klausner D J, Thoen C O, Johnson D W

出版信息

J Clin Microbiol. 1978 Feb;7(2):172-5. doi: 10.1128/jcm.7.2.172-175.1978.

Abstract

Lymphocytes from Mycobacterium bovis-sensitized and normal cattle were cultured in round- and/or flat-bottom microtiter plates and stimulated with M. bovis purified protein derivative (PPD) tuberculin. Blastogenic responses of lymphocytes from M. bovis-sensitized cattle to PPD cultured in round-bottom plates were significantly greater than those of lymphocytes cultured in flat-bottom microtiter plates. Normal lymphocytes of nonsensitized cattle were not stimulated by PPD in either round- or flat-bottom microtiter plates. Kinetics of lymphocyte responses in round-bottom plates are presented.

摘要

将来自牛分枝杆菌致敏牛和正常牛的淋巴细胞培养于圆底和/或平底微量滴定板中,并用牛分枝杆菌纯化蛋白衍生物(PPD)结核菌素进行刺激。来自牛分枝杆菌致敏牛的淋巴细胞对在圆底培养板中培养的PPD的增殖反应显著大于在平底微量滴定板中培养的淋巴细胞。未致敏牛的正常淋巴细胞在圆底或平底微量滴定板中均未受到PPD的刺激。文中给出了圆底培养板中淋巴细胞反应的动力学情况。

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