单纯疱疹病毒和痘苗病毒对致敏淋巴细胞的体外刺激
In vitro stimulation of sensitized lymphocytes by herpes simplex virus and vaccinia virus.
作者信息
Rosenberg G L, Farber P A, Notkins A L
出版信息
Proc Natl Acad Sci U S A. 1972 Mar;69(3):756-60. doi: 10.1073/pnas.69.3.756.
Splenic lymphocytes from rabbits immunized with herpes simplex virus (HSV) were incubated in vitro with ultraviolet light-inactivated HSV, and the degree of lymphocyte transformation was determined by measurement of the incorporation of [(3)H]thymidine into acid-insoluble material. Lymphocytes from immunized rabbits were stimulated as much as 30-fold, whereas lymphocytes from control rabbits were unaffected. Lymphocyte sensitization occurred within 3 days after immunization; sensitized lymphocytes could still be detected 120 days after immunization. The antigenicity of the ultraviolet light-inactivated crude virus pool was found to reside primarily in the virion. Infectious virus, in comparison with inactivated virus, produced less lymphocyte stimulation. Studies on the interaction of the humoral and cellular immune responses showed that incubation of anti-HSV antibody with HSV antigens did not reduce the capacity of the viral antigens to stimulate sensitized lymphocytes. Other experiments revealed that lymphocytes from both the spleen and peripheral blood of animals immunized with vaccinia virus could be stimulated by vaccinia, but not by HSV. Conversely, lymphocytes from animals immunized with HSV could not be stimulated by vaccinia. The transformation of sensitized lymphocytes by viral antigens appears to be a simple, highly specific, and quantitative in vitro technique for the study of the cellular immune response to viral infections.
将用单纯疱疹病毒（HSV）免疫的家兔的脾淋巴细胞，在体外与紫外线灭活的HSV一起孵育，通过测量[（3）H]胸苷掺入酸不溶性物质的量来确定淋巴细胞转化程度。免疫家兔的淋巴细胞受到的刺激高达30倍，而对照家兔的淋巴细胞则未受影响。淋巴细胞致敏在免疫后3天内发生；致敏淋巴细胞在免疫后120天仍可检测到。发现紫外线灭活的粗病毒库的抗原性主要存在于病毒粒子中。与灭活病毒相比，感染性病毒产生的淋巴细胞刺激较少。对体液免疫和细胞免疫反应相互作用的研究表明，抗HSV抗体与HSV抗原孵育不会降低病毒抗原刺激致敏淋巴细胞的能力。其他实验表明，用痘苗病毒免疫的动物的脾和外周血中的淋巴细胞可被痘苗刺激，但不能被HSV刺激。相反，用HSV免疫的动物的淋巴细胞不能被痘苗刺激。病毒抗原对致敏淋巴细胞的转化似乎是一种用于研究对病毒感染的细胞免疫反应的简单、高度特异性和定量的体外技术。
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