Bailly C, Drèze S, Asselineau D, Nusgens B, Lapière C M, Darmon M
Cell Biology Department, Centre International de Recherches Dermatologiques (CIRD), Sophia Antipolis, France.
J Invest Dermatol. 1990 Jan;94(1):47-51. doi: 10.1111/1523-1747.ep12873342.
Lattices made of collagen and fibroblasts can be used as dermal equivalents to grow human keratinocytes in vitro. When these cultures are performed in a medium containing delipidized serum, the lattice is eventually degraded by the growing epithelium. The digestion of the dermal equivalent is due to the secretion of a collagenase by the keratinocytes. This degradation does not occur in cultures containing total serum or supplemented with retinoic acid. We show in this paper that retinoic acid inhibits the secretion of this keratinocyte collagenase in a dose-dependent manner. In the light of this result, the possible involvement of collagenase inhibition in the therapeutic effect of retinoic acid in skin disorders and skin aging must be considered.
由胶原蛋白和成纤维细胞构成的晶格可作为真皮替代物用于体外培养人角质形成细胞。当在含有脱脂血清的培养基中进行这些培养时，晶格最终会被生长的上皮细胞降解。真皮替代物的消化是由于角质形成细胞分泌了一种胶原酶。在含有全血清或补充了视黄酸的培养物中不会发生这种降解。我们在本文中表明，视黄酸以剂量依赖的方式抑制这种角质形成细胞胶原酶的分泌。鉴于这一结果，必须考虑胶原酶抑制在视黄酸对皮肤疾病和皮肤衰老的治疗作用中可能发挥的作用。
