水痘带状疱疹病毒膜相关抗原抗体的酶免疫测定

Enzyme immunoassay for antibodies to membrane associated antigen of varicella zoster virus.

作者信息

Cox J C, Moloney M B, Herrington R W, Hampson A W, Hurrell J G

出版信息

J Virol Methods. 1984 Feb;8(1-2):137-45. doi: 10.1016/0166-0934(84)90047-8.

Abstract

An in situ enzyme immunoassay to viral membrane antigen was developed to enable the specific estimation of antibodies to varicella zoster (VZ) virus. The technique was compared with a modified fluorescent antibody to membrane antigen (FAMA) procedure and with the complement fixation (CF) test by parallel assay of 352 plasma samples. The enzyme immunoassay (EIA) procedure showed very good correlation with the modified FAMA procedure, and both were far more specific than the CF test. This specificity was achieved by the use, in the EIA, of VZ virus-infected cells grown and fixed in situ with glutaraldehyde. Thus the only virus antigens accessible to antibody were the VZ-specific antigens expressed at the cell membrane, cross-reactions with herpes simplex virus antibodies thereby being avoided.

摘要

开发了一种针对病毒膜抗原的原位酶免疫测定法，以能够特异性地估计水痘带状疱疹（VZ）病毒抗体。通过对352份血浆样本进行平行检测，将该技术与改良的膜抗原荧光抗体（FAMA）程序以及补体结合（CF）试验进行了比较。酶免疫测定（EIA）程序与改良的FAMA程序显示出非常好的相关性，并且两者都比CF试验特异性强得多。这种特异性是通过在EIA中使用戊二醛原位生长和固定的VZ病毒感染细胞来实现的。因此，抗体可接触到的唯一病毒抗原是在细胞膜上表达的VZ特异性抗原，从而避免了与单纯疱疹病毒抗体的交叉反应。