Preissner C M, Steinberg S P, Gershon A A, Smith T F
J Clin Microbiol. 1982 Aug;16(2):373-6. doi: 10.1128/jcm.16.2.373-376.1982.
The anticomplement immunofluorescence (ACIF) test was compared with complement fixation and fluorescent antibody to membrane antigen procedures for the detection of antibody to varicella-zoster virus. All of 50 sera from pregnant women contained antibody measured by ACIF (titer, greater than or equal to 1:10); only 27 (54%) were positive by complement fixation (P less than 0.01). For 16 paired sera obtained before and after varicella-zoster virus infection and tested by ACIF and fluorescent antibody to membrane antigen, the result agreed in 27 determinations (sensitivity, 94%; specificity, 81%). Of 99 sera submitted for routine determinations of immune status to the virus, 89 showed comparable results for both tests (sensitivity, 92.5%; specificity, 88%). The ACIF test offers a specific and sensitive alternative to the fluorescent antibody to membrane antigen procedure for the detection of antibody to varicella-zoster virus. In addition, the ACIF test is rapid, easy to perform, and uses commercially available reagents.
将抗补体免疫荧光（ACIF）试验与补体结合试验以及膜抗原荧光抗体试验进行比较，以检测水痘 - 带状疱疹病毒抗体。50份孕妇血清经ACIF检测均含有抗体（效价大于或等于1:10）；补体结合试验仅27份（54%）呈阳性（P<0.01）。对16份水痘 - 带状疱疹病毒感染前后采集的配对血清进行ACIF和膜抗原荧光抗体检测，27次检测结果一致（敏感性94%；特异性81%）。在提交进行该病毒免疫状态常规检测的99份血清中，89份两种检测结果相当（敏感性92.5%；特异性88%）。ACIF试验为检测水痘 - 带状疱疹病毒抗体提供了一种特异性和敏感性均较高的替代膜抗原荧光抗体试验的方法。此外，ACIF试验快速、易于操作，且使用市售试剂。
