Ekblom P, Miettinen M, Forsman L, Andersson L C
J Clin Pathol. 1984 Apr;37(4):357-63. doi: 10.1136/jcp.37.4.357.
The distributions of defined basement membrane proteins in nine pure tubular carcinomas, 10 cases of sclerosing adenosis, and 15 ductal adenocarcinomas were compared. Sections of formalin fixed, paraffin embedded specimens were pretreated with pepsin and then immunostained for laminin, type IV collagen, and basement membrane proteoglycan, components specific for basement membranes. In sclerosing adenosis the tubules were surrounded by a continuous intact basement membrane composed of laminin, type IV collagen, and basement membrane proteoglycan, while the epithelium in the tubular carcinomas was negative for these proteins. The tumours were also analysed for the distribution of the apocrine epithelial antigen (AEA). In contrast to the benign lesions the tubular carcinomas expressed the AEA in a distinct non-polar fashion throughout the cell surface. In normal ducts and in adenosis the AEA was confined exclusively to the luminal surface. These studies suggest that there is a disturbance of cell polarity in tubular carcinomas. It is concluded that a combined analysis of basement membrane proteins and luminal surface antigens is a reliable and convenient way to differentiate between tubular carcinoma and sclerosing adenosis of the breast.
比较了9例纯管状癌、10例硬化性腺病和15例导管腺癌中特定基底膜蛋白的分布情况。将福尔马林固定、石蜡包埋标本的切片用胃蛋白酶预处理，然后进行层粘连蛋白、IV型胶原和基底膜蛋白聚糖的免疫染色，这些都是基底膜特有的成分。在硬化性腺病中，小管被由层粘连蛋白、IV型胶原和基底膜蛋白聚糖组成的连续完整基底膜所包围，而管状癌中的上皮细胞对这些蛋白呈阴性。还分析了肿瘤中顶泌汗腺上皮抗原（AEA）的分布。与良性病变不同，管状癌在整个细胞表面以独特的非极性方式表达AEA。在正常导管和腺病中，AEA仅局限于管腔表面。这些研究表明管状癌中存在细胞极性紊乱。得出结论，对基底膜蛋白和管腔表面抗原进行联合分析是区分乳腺管状癌和硬化性腺病的可靠且便捷的方法。
