Horodyski F M, Holland J J
J Gen Virol. 1984 Apr;65 ( Pt 4):819-23. doi: 10.1099/0022-1317-65-4-819.
Mutants of vesicular stomatitis virus have previously been isolated whose replication is not affected by the presence of defective interfering (DI) particles. We describe here reconstruction experiments in BHK-21 cells which show that DI particle-resistant viruses are strongly selected over wild-type virus in the presence of DI particles. This occurs both during undiluted lytic passage and persistent infection. This selection is evident even when the mutant virus is added initially at very low levels relative to wild-type virus. This indicates that DI particles can be important selective factors affecting viral evolution.
水泡性口炎病毒的突变体先前已被分离出来，其复制不受缺陷干扰（DI）颗粒存在的影响。我们在此描述了在BHK - 21细胞中的重建实验，这些实验表明，在存在DI颗粒的情况下，抗DI颗粒的病毒比野生型病毒更受强烈选择。这在未稀释的裂解传代和持续感染过程中都会发生。即使最初以相对于野生型病毒非常低的水平添加突变病毒，这种选择也是明显的。这表明DI颗粒可能是影响病毒进化的重要选择因素。
