序列导向和蛋白质诱导的DNA弯曲位点。

The locus of sequence-directed and protein-induced DNA bending.

作者信息

Wu H M, Crothers D M

出版信息

Nature. 1984;308(5959):509-13. doi: 10.1038/308509a0.

Abstract

The bending locus of trypanosome kinetoplast DNA, identified by gel electrophoresis, has tracts of a simple repeat sequence (CA5-6 T) symmetrically distributed about it, with a repeat interval of 10 base pairs. The analogous bending induced when catabolite gene activating protein binds to its recognition sequence near the promoter of the Escherichia coli lac operon is centred on a site about 5-7 base pairs away from the centre of the protein binding site.

摘要

通过凝胶电泳鉴定的锥虫动质体DNA的弯曲轨迹，有一段简单重复序列（CA5-6 T）的片段围绕其对称分布，重复间隔为10个碱基对。当分解代谢物基因激活蛋白结合到大肠杆菌乳糖操纵子启动子附近的识别序列时诱导产生的类似弯曲，集中在一个距离蛋白质结合位点中心约5-7个碱基对的位点上。