DNA聚合酶与静息猴细胞的猿猴病毒40感染：抗阿非科林α聚合酶的诱导

DNA polymerase and simian virus 40 infection of resting monkey cells: induction of aphidicolin resistant alpha-polymerase.

作者信息

Tanabe K, Karawya E, Fewell J W, Kuff E L, Wilson S H

出版信息

Nucleic Acids Res. 1983 Dec 10;11(23):8253-68. doi: 10.1093/nar/11.23.8253.

DOI:10.1093/nar/11.23.8253

PMID:6324079

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC326579/

Abstract

Tightly confluent monkey cell lines BSC-1 and CV-1 held in stale medium for several days exhibited an extremely low level of thymidine incorporation into cellular DNA. Yet, these cells contained a level of alpha-polymerase equal to about 15% of the level in rapidly dividing cells, and they still were capable of supporting replication of SV40 DNA. SV40 infection and culture in stale medium resulted in a four-fold induction of alpha-polymerase in CV-1 cells, whereas no change in alpha-polymerase level was observed in BSC-1 cells. Characterization of alpha-polymerase partially purified from infected CV-1 cells revealed that 80-90% of the enzyme activity was aphidicolin resistant. SV40 DNA replication in resting CV-1 cells, however, was aphidicolin sensitive. SV40 infection of resting CV-1 cells may induce an aphidicolin-resistant enzyme or lead to a modified alpha-polymerase species.

摘要

在陈旧培养基中培养数天的紧密汇合的猴细胞系BSC - 1和CV - 1，其胸苷掺入细胞DNA的水平极低。然而，这些细胞中的α - 聚合酶水平约为快速分裂细胞中水平的15%，并且它们仍然能够支持SV40 DNA的复制。在陈旧培养基中进行SV40感染和培养导致CV - 1细胞中的α - 聚合酶诱导增加四倍，而在BSC - 1细胞中未观察到α - 聚合酶水平的变化。对从感染的CV - 1细胞中部分纯化的α - 聚合酶的特性分析表明，80 - 90%的酶活性对阿非科林具有抗性。然而，静止的CV - 1细胞中的SV40 DNA复制对阿非科林敏感。静止的CV - 1细胞的SV40感染可能诱导一种对阿非科林具有抗性的酶，或导致一种经过修饰的α - 聚合酶种类。