Department of Biochemistry, Cancer Institute, Japanese Foundation for Cancer Research, Toshima-ku, Tokyo 170, Japan.
Proc Natl Acad Sci U S A. 1981 Sep;78(9):5305-9. doi: 10.1073/pnas.78.9.5305.
We have cloned and analyzed a chromosomal DNA segment containing the human interferon beta(1) gene from a human gene library. The nucleotide sequence of the protein-coding and the noncoding regions of the chromosomal gene was identical to the cDNA sequence reported previously. In the region upstream from the putative transcription initiation site, significant nucleotide sequence homology was observed between interferon beta(1) and alpha(1) genes. This region thus may play a role in expression of the interferon genes. From the sequence data and the result of nuclease S1 mapping experiments, we conclude that, like the interferon alpha(1) gene, the interferon beta(1) gene is devoid of intervening sequences.
我们已经从人类基因文库中克隆并分析了一段含有人类干扰素β(1)基因的染色体 DNA 片段。染色体基因的蛋白编码区和非编码区的核苷酸序列与先前报道的 cDNA 序列完全相同。在假定的转录起始位点上游区域，干扰素β(1)和α(1)基因之间观察到显著的核苷酸序列同源性。因此，该区域可能在干扰素基因的表达中发挥作用。根据序列数据和核酸酶 S1 作图实验的结果，我们得出结论，与干扰素α(1)基因一样，干扰素β(1)基因不含内含子。
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