pFJ265, a new cloning vehicle for Streptomyces.
作者信息
Jones M D, Mabe J A, Nakatsukasa W M, Fayerman J T
出版信息
Plasmid. 1984 Jan;11(1):92-5. doi: 10.1016/0147-619x(84)90011-8.
PMID:6324262
Abstract
A 9.3-kb plasmid, pNM100, was isolated from Streptomyces virginiae (NRRL 15156) and characterized. Streptomyces genes for thiostrepton and neomycin resistance were cloned into pNM100 to yield a small plasmid derivative, pFJ265, that is suitable for Streptomyces gene cloning. pFJ265 is a 9.2-kb nonconjugative plasmid and has a copy number of several hundred per chromosome.
摘要
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