通过完全或特定部分乙酰亚胺化对细胞色素c和细胞色素c-T中碱性转变的调节。
Modulation of the alkaline transition in cytochrome c and cytochrome c-T by full or specific partial acetimidylation.
作者信息
Wallace C J
出版信息
Biochem J. 1984 Feb 1;217(3):601-4. doi: 10.1042/bj2170601.
Abstract
Acetimidylated horse cytochrome c and related derivatives exhibit more or less marked changes, both upscale and downscale, in apparent pK of the alkaline transition. This transition occurs when the normal methionine-80 residue is replaced at the sixth haem co-ordination position by another strong-field ligand. Analysis of the relationship between structural change and pK shift in these derivatives supports the view that the replacement ligand is a lysine residue, probably 72 or 79, and contradicts an alternative hypothesis. The results add further detail to a comprehensive view of the mechanism of this isomerization.
摘要
乙酰亚胺化马细胞色素c及相关衍生物在碱性转变的表观pK值上或多或少呈现出明显的变化，既有升高也有降低。当正常的甲硫氨酸-80残基在第六个血红素配位位置被另一个强场配体取代时，就会发生这种转变。对这些衍生物中结构变化与pK位移之间关系的分析支持了这样一种观点，即取代配体是一个赖氨酸残基，可能是72或79，这与另一种假设相矛盾。这些结果为这种异构化机制的全面观点增添了更多细节。
相似文献
1
Modulation of the alkaline transition in cytochrome c and cytochrome c-T by full or specific partial acetimidylation.通过完全或特定部分乙酰亚胺化对细胞色素c和细胞色素c-T中碱性转变的调节。
Biochem J. 1984 Feb 1;217(3):601-4. doi: 10.1042/bj2170601.
2
The effect of complete or specific partial acetimidylation on the biological properties of cytochrome c and cytochrome c-T.完全或特定部分乙酰亚胺化对细胞色素c和细胞色素c-T生物学特性的影响。
Biochem J. 1984 Feb 1;217(3):595-9. doi: 10.1042/bj2170595.
3
The preparation of fully N-epsilon-acetimidylated cytochrome c.全N-ε-乙酰亚胺化细胞色素c的制备
Biochem J. 1984 Feb 1;217(3):589-94. doi: 10.1042/bj2170589.
4
Alkaline isomerization of thermoresistant cytochrome c-552 and horse heart cytochrome c studied by absorption and resonance Raman spectroscopy.通过吸收光谱和共振拉曼光谱研究耐热细胞色素c-552和马心脏细胞色素c的碱性异构化。
Biochim Biophys Acta. 1978 Feb 15;532(2):337-46. doi: 10.1016/0005-2795(78)90588-3.
5
Involvement of lysines-72 and -79 in the alkaline isomerization of horse heart ferricytochrome c.赖氨酸-72和-79参与马心铁细胞色素c的碱异构化反应。
Biochemistry. 1980 Mar 18;19(6):1117-20. doi: 10.1021/bi00547a012.
6
Alkaline isomerization of ferricytochrome c: identification of the lysine ligand.高铁细胞色素c的碱性异构化：赖氨酸配体的鉴定
Proc Natl Acad Sci U S A. 1974 Jul;71(7):2892-4. doi: 10.1073/pnas.71.7.2892.
7
The binding characteristics of the cytochrome c iron.细胞色素c铁的结合特性
Biochem J. 1988 Oct 1;255(1):353-6.
8
The semisynthesis of fragments corresponding to residues 66-104 of horse heart cytochrome c.马心脏细胞色素c中对应于66 - 104位残基片段的半合成。
Biochem J. 1979 Apr 1;179(1):169-82. doi: 10.1042/bj1790169.
9
Bacterial expression of a mitochondrial cytochrome c. Trimethylation of lys72 in yeast iso-1-cytochrome c and the alkaline conformational transition.线粒体细胞色素c的细菌表达。酵母同工-1-细胞色素c中赖氨酸72的三甲基化与碱性构象转变。
Biochemistry. 1998 Apr 28;37(17):6124-31. doi: 10.1021/bi972188d.
10
Haem ligands of the ferricytochrome c of Ustilago sphaerogena.黑粉菌铁细胞色素c的血红素配体。
Biochem J. 1974 Jun;139(3):547-53. doi: 10.1042/bj1390547.
引用本文的文献
1
Structural and thermodynamic behavior of cytochrome c assembled with glutathione-covered gold nanoparticles.与谷胱甘肽包覆的金纳米颗粒组装的细胞色素c的结构和热力学行为。
J Biol Inorg Chem. 2009 May;14(4):621-30. doi: 10.1007/s00775-009-0476-x. Epub 2009 Feb 11.
2
The preparation of fully N-epsilon-acetimidylated cytochrome c.全N-ε-乙酰亚胺化细胞色素c的制备
Biochem J. 1984 Feb 1;217(3):589-94. doi: 10.1042/bj2170589.
3
A new non-covalent complex of semisynthetically modified tryptic fragments of cytochrome c.一种细胞色素c半合成修饰胰蛋白酶片段的新型非共价复合物。
Biochem J. 1986 Oct 15;239(2):333-7. doi: 10.1042/bj2390333.
4
On the relationship between oxidation-reduction potential and biological activity in cytochrome c analogues. Results from four novel two-fragment complexes.关于细胞色素c类似物中氧化还原电位与生物活性之间的关系。来自四种新型双片段复合物的结果。
Biochem J. 1987 Aug 1;245(3):773-9. doi: 10.1042/bj2450773.
本文引用的文献
1
The effect of complete or specific partial acetimidylation on the biological properties of cytochrome c and cytochrome c-T.完全或特定部分乙酰亚胺化对细胞色素c和细胞色素c-T生物学特性的影响。
Biochem J. 1984 Feb 1;217(3):595-9. doi: 10.1042/bj2170595.
2
The preparation of fully N-epsilon-acetimidylated cytochrome c.全N-ε-乙酰亚胺化细胞色素c的制备
Biochem J. 1984 Feb 1;217(3):589-94. doi: 10.1042/bj2170589.
3
The conformation of eukaryotic cytochrome c around residues 39, 57, 59 and 74.真核细胞色素c在39、57、59和74位残基附近的构象。
Biochem J. 1983 Sep 1;213(3):687-700. doi: 10.1042/bj2130687.
4
Ionization of tyrosine and lysine residues in native and modified horse cytochrome c.天然和修饰的马细胞色素c中酪氨酸和赖氨酸残基的电离作用
Biochem J. 1983 Sep 1;213(3):679-86. doi: 10.1042/bj2130679.
5
Alkaline isomerization of ferricytochrome c: lysine is not replacing methionine at the sixth co-ordination site of the haem iron.高铁细胞色素c的碱性异构化：赖氨酸不会取代血红素铁第六配位位点上的甲硫氨酸。
J Mol Biol. 1981 Dec 25;153(4):1125-49. doi: 10.1016/0022-2836(81)90471-x.
6
N.m.r. and e.p.r. characterization of [4-carboxy-2,6-dinitrophenyl-lysine]cytochromes c.[4-羧基-2,6-二硝基苯基-赖氨酸]细胞色素c的核磁共振和电子顺磁共振表征
Biochem J. 1981 Mar 1;193(3):1021-4. doi: 10.1042/bj1931021.
7
Involvement of lysines-72 and -79 in the alkaline isomerization of horse heart ferricytochrome c.赖氨酸-72和-79参与马心铁细胞色素c的碱异构化反应。
Biochemistry. 1980 Mar 18;19(6):1117-20. doi: 10.1021/bi00547a012.
8
9
Conformation of ferricytochrome c. IV. Relationship between optical absorption and protein conformation.高铁细胞色素c的构象。IV. 光吸收与蛋白质构象之间的关系。
Biopolymers. 1967;5(8):788-90. doi: 10.1002/bip.1967.360050812.
10
The existence of heme-protein coordinate-covalent bonds in denaturing solvents.变性溶剂中血红素 - 蛋白质配位共价键的存在。
Biopolymers. 1971 Nov;10(11):2359-61. doi: 10.1002/bip.360101125.
Zotero 插件