Smith R J, Bowman B J, Iden S S
Biochem Pharmacol. 1984 Apr 1;33(7):973-8. doi: 10.1016/0006-2952(84)90502-1.
1-O-Hexadecyl/octadecyl-2-acetyl-sn-glyceryl-3-phosphorylcholine (AGEPC) stimulated a time- and concentration-dependent generation of superoxide anion (O-2) by human neutrophils. Maximum production of O-2 occurred 60 sec subsequent to cell contact with AGEPC. Superoxide generation was reduced significantly if cells were not preincubated with cytochalasin B prior to exposure to AGEPC (0.01 to 10 microM). A time-dependent desensitization for O-2 production was demonstrated in neutrophilis which were stimulated with AGEPC prior to contact with cytochalasin B. The rate and amount of O-2 generated by AGEPC-activated neutrophils were enhanced significantly in the presence of extra-cellular calcium. However, incubation of neutrophils with ethyleneglycol-bis(beta-amino-ethyl ether) N,N'-tetra-acetate (EGTA) in calcium-free medium had no effect on the O-2 generating system. AGEPC-induced O-2 production was suppressed by the glycolytic inhibitor 2-deoxy-D-glucose (2-DG) and the sulfhydryl reagents N-ethylmaleimide (NEM) and iodoacetic acid (1A). Sodium cyanide was inactive. Pretreatment of neutrophils with AGEPC reduced cell responsiveness to subsequent exposure to this stimulus. Desensitization of the O-2-generating system activated with AGEPC appears to be stimulus specific for leukotriene B4 (LTB4) and N-formyl-methionyl-leucyl-phenylalanine (FMLP) in that these stimuli were capable of inducing O-2 production by cells pretreated with AGEPC. However, neutrophils pretreated with AGEPC were desensitized to zymosan-activated serum (ZAS).
1-十六烷基/十八烷基-2-乙酰基-sn-甘油-3-磷酸胆碱（AGEPC）刺激人中性粒细胞产生超氧阴离子（O₂⁻），呈现时间和浓度依赖性。细胞与AGEPC接触后60秒时O₂⁻产量达到最大值。如果细胞在暴露于AGEPC（0.01至10微摩尔）之前未用细胞松弛素B预孵育，超氧阴离子的产生会显著减少。在用AGEPC刺激后再与细胞松弛素B接触的中性粒细胞中，显示出对O₂⁻产生的时间依赖性脱敏现象。在细胞外钙存在的情况下，AGEPC激活的中性粒细胞产生O₂⁻的速率和量显著增加。然而，在无钙培养基中用乙二醇双（β-氨基乙醚）N，N'-四乙酸（EGTA）孵育中性粒细胞对O₂⁻产生系统没有影响。AGEPC诱导的O₂⁻产生受到糖酵解抑制剂2-脱氧-D-葡萄糖（2-DG）以及巯基试剂N-乙基马来酰亚胺（NEM）和碘乙酸（1A）的抑制。氰化钠无活性。用AGEPC预处理中性粒细胞会降低细胞对随后暴露于该刺激的反应性。由AGEPC激活的O₂⁻产生系统的脱敏似乎对白三烯B4（LTB4）和N-甲酰甲硫氨酰亮氨酰苯丙氨酸（FMLP）具有刺激特异性，因为这些刺激能够诱导用AGEPC预处理的细胞产生O₂⁻。然而，用AGEPC预处理的中性粒细胞对酵母聚糖激活的血清（ZAS）脱敏。
