Wagner J C, Griffiths D M, Hill R J
Br J Cancer. 1984 Apr;49(4):453-8. doi: 10.1038/bjc.1984.72.
Standard (UICC) crocidolite was subjected to ball milling to reduce the length of the fibre. These milled materials and the original standard sample were injected into the pleural cavity of rats to determine their ability to induce mesothelioma. Previous in vitro work on the same materials had suggested that biological activity was related to fibres greater than 6.5 microns in length and that the material milled for 4 and 8 h did not contain fibres in this range and was biologically inactive. The results of the animal work, however, did not follow this pattern; mesotheliomas occurred in rats in all treatment groups including the 4 and 8 h milled samples. Examination of the tissues and the dust recovered from them showed the presence of fibres greater than the suggested threshold. Attention is drawn to the problems associated with drawing conclusions from size distributions and in vitro studies without considering in vivo mechanisms.
对标准（国际癌症研究机构）青石棉进行球磨以缩短纤维长度。将这些研磨后的材料和原始标准样品注入大鼠胸腔，以确定它们诱发间皮瘤的能力。之前对相同材料的体外研究表明，生物活性与长度大于6.5微米的纤维有关，并且研磨4小时和8小时的材料在此范围内不含纤维且无生物活性。然而，动物实验结果并未遵循这一模式；所有治疗组的大鼠都发生了间皮瘤，包括研磨4小时和8小时的样品。对组织和从中回收的粉尘进行检查发现存在大于建议阈值的纤维。需要注意的是，在不考虑体内机制的情况下，从尺寸分布和体外研究得出结论存在相关问题。
