诱导进行神经元分化的胚胎癌细胞系中的离子通道。
Ionic channels in a line of embryonal carcinoma cells induced to undergo neuronal differentiation.
作者信息
Ebihara L, Speers W C
出版信息
Biophys J. 1984 Dec;46(6):827-30. doi: 10.1016/S0006-3495(84)84081-3.
Abstract
The gigaseal patch clamp technique was used to investigate the electrophysiological properties of a line of embryonal carcinoma cells (PCC4) that were induced to undergo neuronal differentiation. A large increase in number of voltage-dependent potassium and sodium channels was observed during differentiation. The pharmacology and kinetics of the macroscopic sodium and potassium currents in the differentiated cells closely resembled those of the rapid inward sodium current and the delayed rectifier, respectively. The kinetic behavior of single-channel potassium currents was consistent with the properties of the macroscopic delayed rectifier current.
摘要
采用千兆封接膜片钳技术研究了诱导分化为神经元的胚胎癌细胞系（PCC4）的电生理特性。在分化过程中观察到电压依赖性钾通道和钠通道数量大幅增加。分化细胞中宏观钠电流和钾电流的药理学和动力学特性分别与快速内向钠电流和延迟整流器的特性极为相似。单通道钾电流的动力学行为与宏观延迟整流器电流的特性一致。
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