Hoover R L, Karnovsky M J, Austen K F, Corey E J, Lewis R A
Proc Natl Acad Sci U S A. 1984 Apr;81(7):2191-3. doi: 10.1073/pnas.81.7.2191.
The attachment of human polymorphonuclear leukocytes (PMN) to bovine endothelial monolayers is increased by pretreatment of the endothelial cells with leukotriene B4 (LTB4). The response is dose dependent with a maximum at 10(-6) M, requires no more than 1 min of preincubation for the maximal effect, and decays linearly over 30 min once the LTB4 is removed. Preincubation of PMN with LTB4 does not augment subsequent adhesion to a plastic substratum or an untreated monolayer of endothelial cells and eliminates the augmented attachment to LTB4-pretreated endothelial cells. The simultaneous exposure of both cell types during the adhesion interaction gives a biphasic dose-related increment, suggesting that the inhibition of the neutrophil adherence is less marked than with a pretreatment interval. Thus, the LTB4-dependent adherence of PMN to endothelial cells is selectively mediated by endothelial cells, is dose related, is reversible upon removal of the agonist from the endothelial cells, and is opposed by the direct action of LTB4 on the PMN.
用白三烯B4（LTB4）预处理内皮细胞可增强人多形核白细胞（PMN）与牛内皮细胞单层的黏附。该反应呈剂量依赖性，在10^(-6) M时达到最大值，最大效应所需的预孵育时间不超过1分钟，一旦去除LTB4，其效应在30分钟内呈线性衰减。用LTB4预孵育PMN不会增强其随后与塑料基质或未处理的内皮细胞单层的黏附，且会消除其与LTB4预处理的内皮细胞增强的黏附。在黏附相互作用期间同时暴露两种细胞类型会产生双相剂量相关的增加，这表明中性粒细胞黏附的抑制作用不如预处理间隔时明显。因此，PMN与内皮细胞的LTB4依赖性黏附是由内皮细胞选择性介导的，呈剂量相关，在内皮细胞中去除激动剂后是可逆的，并且受到LTB4对PMN的直接作用的拮抗。
