Müller H, Harmuth-Hoene A E
Z Ernahrungswiss. 1984 Mar;23(1):52-7. doi: 10.1007/BF02020896.
A simple method is described by which the fecal flora of rats has been isolated with high recovery and a high purity grade. Total nitrogen and 2.6-diamino-pimelic acid were determined in the isolated bacteria. From both values a factor was calculated, which permits the estimation of fecal bacterial nitrogen from fecal DAP content. In the feces of rats on a semisynthetic control diet (4% cellulose) this factor was 15.7. Addition of 8% guar (by substitution for starch) resulted in a drastic increase in fecal DAP content thus lowering the factor to 11.5. When the proportion of cellulose in the control diet was raised from 4 to 12%, no change in the factor was observed. This indicates a pronounced effect on the bacterial activity by guar but not by cellulose. It is concluded that the factor used to calculate fecal microbial nitrogen from fecal DAP content is dependent on the kind of dietary fiber ingested.
本文描述了一种简单的方法，通过该方法可高回收率、高纯度地分离大鼠粪便菌群。对分离出的细菌进行了总氮和2,6-二氨基庚二酸的测定。根据这两个值计算出一个因子，该因子可用于根据粪便中2,6-二氨基庚二酸的含量估算粪便细菌氮含量。在半合成对照饮食（含4%纤维素）的大鼠粪便中，该因子为15.7。添加8%瓜尔豆胶（替代淀粉）会导致粪便中2,6-二氨基庚二酸含量大幅增加，从而使该因子降至11.5。当对照饮食中纤维素的比例从4%提高到12%时，该因子未观察到变化。这表明瓜尔豆胶对细菌活性有显著影响，而纤维素则没有。得出的结论是，用于根据粪便中2,6-二氨基庚二酸含量计算粪便微生物氮的因子取决于摄入的膳食纤维种类。
