Fadly A M, Nazerian K
Avian Dis. 1984 Jan-Mar;28(1):183-96.
Poults free from hemorrhagic enteritis (HE) antibody were vaccinated by gavage at 1 day or 2 weeks of age with a live HE vaccine virus that had been propagated in a Marek's disease (MD)-induced B-lymphoblastoid cell line of turkey origin. Vaccinated and unvaccinated poults were challenged with a virulent HE virus at various times postvaccination. One hundred tissue-culture-infectious doses of the vaccine virus per poult were sufficient to induce a serological response as well as to protect poults against HE lesions and mortality. Vaccinated poults were protected against the disease as early as 1 week and as late as 8 weeks PV. The vaccine was efficacious by several routes of application. The vaccine virus spread horizontally from vaccinated to contact-exposed poults, as indicated by seroconversion and resistance of contact-exposed poults to challenge. The vaccine had no detectable harmful effects on the humoral immune response to particulate antigens or on weight gain of vaccinated poults. The vaccine proved to be free from MD virus, as indicated by the absence of MD lesions and antibody in 8-week-old chickens inoculated intra-abdominally with the vaccine at hatching. These findings indicate that the cell-culture-propagated HE vaccine is efficacious and safe.
将无出血性肠炎（HE）抗体的雏禽在1日龄或2周龄时通过灌胃接种在源自火鸡的马立克氏病（MD）诱导的B淋巴细胞系中传代培养的活HE疫苗病毒。在接种疫苗后的不同时间，对接种和未接种的雏禽用强毒HE病毒进行攻毒。每只雏禽接种100个组织培养感染剂量的疫苗病毒足以诱导血清学反应，并保护雏禽免受HE病变和死亡。接种疫苗的雏禽最早在接种后1周、最晚在接种后8周受到疾病保护。该疫苗通过几种接种途径均有效。疫苗病毒从接种疫苗的雏禽横向传播到接触暴露的雏禽，这表现为接触暴露雏禽的血清转化和对攻毒的抵抗力。该疫苗对颗粒抗原的体液免疫反应或对接种疫苗雏禽的体重增加没有可检测到的有害影响。如在孵化时腹腔内接种该疫苗的8周龄鸡未出现MD病变和抗体所示，该疫苗被证明不含MD病毒。这些发现表明，细胞培养传代的HE疫苗是有效且安全的。
