Emami S, Perry M C
Biochim Biophys Acta. 1984 May 22;804(1):77-88. doi: 10.1016/0167-4889(84)90101-0.
Sodium nitroprusside, a known activator of guanylate cyclase within cells, was used as a probe to investigate the possible role of cyclic GMP in the control of metabolism within rat isolated white adipocytes. Over the concentration range 0-0.1 mM, it increased intracellular cyclic GMP concentrations up to 6-fold within 2 min. Over the same concentration range, it increased the incorporation of 14C from D-[U-14C]glucose into triacylglycerol and of L-[14C]leucine into protein. It also inhibited adrenalin -stimulated lipolysis in the cells, but had no effect on the transport of glucose into the cells. The effects of sodium nitroprusside were compared with those elicited by insulin under identical conditions, as this hormone was shown to cause a similar, but transient, rise in intracellular cyclic GMP concentrations within these cells. Nor insulin, neither sodium nitroprusside were able to increase cyclic AMP levels in adipocytes, whereas adrenalin (0.3 microM) stimulated this production. It is suggested that cyclic GMP may have a role in the control of some part of metabolism 'glucose or amino acids' in adipocytes, and that sodium nitroprusside is a useful probe to investigate this. The limitation of its use are discussed.
硝普钠是一种已知的细胞内鸟苷酸环化酶激活剂，被用作探针来研究环磷酸鸟苷（cGMP）在大鼠分离的白色脂肪细胞代谢控制中的可能作用。在0 - 0.1 mM的浓度范围内，它在2分钟内使细胞内cGMP浓度增加高达6倍。在相同浓度范围内，它增加了D-[U-¹⁴C]葡萄糖中¹⁴C掺入三酰甘油以及L-[¹⁴C]亮氨酸掺入蛋白质的量。它还抑制细胞中肾上腺素刺激的脂肪分解，但对葡萄糖进入细胞的转运没有影响。在相同条件下，将硝普钠的作用与胰岛素引发的作用进行了比较，因为已表明这种激素会使这些细胞内的cGMP浓度出现类似但短暂的升高。胰岛素和硝普钠均不能增加脂肪细胞中环磷酸腺苷（cAMP）水平，而肾上腺素（0.3 μM）刺激了cAMP的产生。提示cGMP可能在脂肪细胞中“葡萄糖或氨基酸”代谢的某些部分控制中起作用，并且硝普钠是研究这一作用的有用探针。文中讨论了其使用的局限性。
