Braughler J M, Maron M B
Eur J Pharmacol. 1982 Feb 26;78(2):187-93. doi: 10.1016/0014-2999(82)90235-7.
The intact, isolated perfused dog lung was evaluated as a model for studies directed at defining the role of oxidative modulation of lung cyclic GMP metabolism in pulmonary function. Sodium nitroprusside added to the perfusion blood increased the cyclic GMP content of lung over 4-fold in a dose-dependent manner. Although sodium nitroprusside administration caused changes in lung vascular resistance, these occurred independently of the changes in cyclic GMP. Ventilation of lungs with a high oxygen gas mixture containing 95% O2. 5% CO2 acutely increased the cyclic GMP content of lungs after 15 min from 1.3 +/- 0.06 (mean +/- SE) to 3.4 +/- 0.12 pmol cyclic GMP/mg protein. Cyclic GMP levels returned toward control during continued ventilation with the high oxygen concentration. The oxygen-induced elevation of lung cyclic GMP content was not accompanied by changes in lung vascular resistance. The results indicate that the isolated perfused lung may be useful in studies of cyclic GMP, tissue oxidation and pulmonary function.
完整的离体灌流犬肺被评估为一种模型，用于开展旨在确定肺环鸟苷酸（cGMP）代谢的氧化调节在肺功能中所起作用的研究。添加到灌流血液中的硝普钠可使肺的cGMP含量以剂量依赖的方式增加4倍以上。尽管给予硝普钠会引起肺血管阻力的变化，但这些变化与cGMP的变化无关。用含95% O₂、5% CO₂的高氧气体混合物对肺进行通气，15分钟后肺的cGMP含量从1.3±0.06（平均值±标准误）皮摩尔cGMP/毫克蛋白质急剧增加至3.4±0.12。在持续用高氧浓度通气期间，cGMP水平恢复至对照水平。氧诱导的肺cGMP含量升高并未伴有肺血管阻力的变化。结果表明，离体灌流肺可能有助于cGMP、组织氧化和肺功能的研究。
