Cushman S W, Salans L B
J Lipid Res. 1978 Feb;19(2):269-73.
The osmic acid fixation-Coulter electronic counter method described for determining adipose cell size and number in intact adipose tissue fragments has been modified for use with suspensions of isolated rat and human adipose cells. Mean cell sizes in tissue fragments and isolated cell suspensions prepared from the same tissue are virtually identical in rats of various weights. No statistically significant difference in mean adipose cell size between tissue and isolated cell suspension was observed in human adipose tissue although the variability was much greater than in rat tissue. The distribution of cell sizes among replicate samples is more uniform in the isolated cell preparations, possibly reflecting the considerably larger quantities of tissue used in preparing isolated cells than in determining cell size and number directly from tissue fragments. An example of the utility of the modified method during routine metabolic studies with isolated rat epididymal adipose cells is described; isolated cells of increasing size can be obtained from rats of increasing body weight, or from the separated distal and proximal portions of the fat pads of rats of the same weight.
为测定完整脂肪组织碎片中脂肪细胞大小和数量而描述的锇酸固定 - 库尔特电子计数器法已作修改，用于分离的大鼠和人脂肪细胞悬液。在不同体重的大鼠中，由相同组织制备的组织碎片和分离细胞悬液中的平均细胞大小实际上是相同的。在人脂肪组织中，虽然变异性比大鼠组织大得多，但未观察到组织和分离细胞悬液之间平均脂肪细胞大小有统计学上的显著差异。在分离细胞制剂中，重复样本间细胞大小的分布更均匀，这可能反映出制备分离细胞所用的组织量比直接从组织碎片测定细胞大小和数量时要多得多。描述了该改良方法在对分离的大鼠附睾脂肪细胞进行常规代谢研究中的应用实例；可以从体重增加的大鼠，或从相同体重大鼠脂肪垫分离出的远端和近端部分获得大小不断增加的分离细胞。
