Shulman M J, Hawley R G, Ochi A, Baczynsky W O, Collins C, Pennell N, Potash M J, Köhler G, Hozumi N
Can J Biochem Cell Biol. 1984 Apr;62(4):217-24. doi: 10.1139/o84-031.
Using a mouse hybridoma system, we have developed methods of isolating a variety of mutant cell lines in which immunoglobulin function or synthesis is defective. The analysis of mutants defective in kappa chain synthesis has defined a class of murine transposons. The deletion mutants produce immunoglobulin M (IgM) bearing mu heavy chain fragments and provide information on the requirements of IgM assembly and mu gene expression. We also describe a transfer system for the mu and kappa genes which will be useful in analyzing the structural basis of IgM function.
利用小鼠杂交瘤系统，我们开发了多种分离突变细胞系的方法，这些细胞系中的免疫球蛋白功能或合成存在缺陷。对κ链合成缺陷的突变体分析定义了一类小鼠转座子。缺失突变体产生带有μ重链片段的免疫球蛋白M（IgM），并提供有关IgM组装和μ基因表达要求的信息。我们还描述了一种用于μ和κ基因的转移系统，这将有助于分析IgM功能的结构基础。
