将克隆的免疫球蛋白重链和轻链基因转染至淋巴细胞后功能性免疫球蛋白M的产生。
Functional immunoglobulin M production after transfection of cloned immunoglobulin heavy and light chain genes into lymphoid cells.
作者信息
Ochi A, Hawley R G, Hawley T, Shulman M J, Traunecker A, Köhler G, Hozumi N
出版信息
Proc Natl Acad Sci U S A. 1983 Oct;80(20):6351-5. doi: 10.1073/pnas.80.20.6351.
Abstract
The rearranged immunoglobulin heavy (mu) and light (kappa) chain genes cloned from the Sp6 hybridoma cell line producing immunoglobulin M specific for the hapten 2,4, 6-trinitrophenyl were inserted into the transfer vector pSV2-neo and introduced into various plasmacytoma and hybridoma cell lines. The transfer of the mu and kappa genes resulted in the production of pentameric, hapten-specific, functional IgM.
摘要
从产生对2,4,6-三硝基苯半抗原具有特异性的免疫球蛋白M的Sp6杂交瘤细胞系中克隆出重排的免疫球蛋白重链（μ链）和轻链（κ链）基因，将其插入转移载体pSV2-neo中，并导入各种浆细胞瘤和杂交瘤细胞系。μ链和κ链基因的转移导致了五聚体、半抗原特异性、功能性IgM的产生。
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