Salkoff L
Cold Spring Harb Symp Quant Biol. 1983;48 Pt 1:221-31. doi: 10.1101/sqb.1983.048.01.025.
These genetic and voltage-clamp studies have produced evidence that strongly supports the hypothesis that the Shaker locus codes for at least one molecular component of the IA channel. Mutations at the Shaker locus have effects that are specific to IA, and thus a general membrane component is not produced by this locus. Regarding the function of the Shaker product itself, there is evidence that it is at least involved in the inactivation and recovery process of the channel. The evidence so far is also consistent with there being a single Shaker product per channel. Regarding its genetic and developmental properties, IA is apparently completely independent of the other major voltage-activated K+ channel in the membrane, IK. However, only after molecular information becomes available will it become clear whether or not these experiments have revealed clues about the molecular nature of these channels. Ultimately, voltage-clamp experiments, even combined with resourceful genetic tools, can only reveal circumstantial information about the molecular structure of channels. It is hoped, however, that these studies have stimulated interest in the Shaker locus and that the way has been well prepared for molecular studies of the IA channel. The actual molecular characterization of the Shaker locus is already under way (cf. Jan et al., this volume; M. Tanouye , pers. comm .).
这些遗传学和电压钳研究已产生证据，有力支持了这样的假说：即Shaker基因座编码IA通道的至少一种分子成分。Shaker基因座的突变具有对IA特异的效应，因此该基因座不产生一般的膜成分。关于Shaker产物本身的功能，有证据表明它至少参与通道的失活和恢复过程。目前的证据也与每个通道有单一的Shaker产物相一致。关于其遗传学和发育特性，IA显然完全独立于膜中的另一种主要电压激活钾通道IK。然而，只有在获得分子信息之后，才能清楚这些实验是否揭示了有关这些通道分子性质的线索。最终，电压钳实验，即使与巧妙的遗传工具相结合，也只能揭示有关通道分子结构的间接信息。然而，希望这些研究激发了对Shaker基因座的兴趣，并为IA通道的分子研究做好了充分准备。对Shaker基因座的实际分子表征已经在进行中（参见Jan等人，本卷；M. Tanouye，个人通信）。
