Defreese J D, Wang T S
Crit Rev Clin Lab Sci. 1984;19(4):267-96. doi: 10.3109/10408368409165765.
The accurate determination of the types and amounts of bilirubin species in serum is important for diagnostic purposes as well as for therapeutic monitoring. However, of the determinations routinely performed in the clinical laboratory, those for bilirubins are not among the more accurate and exhibit significant method variability. In this review, the structural, stability, solubility, and albumin-binding properties of serum bilirubins are discussed with respect to their impact on analytical methods. Following a consideration of analytical standards, methods for the determination of unconjugated and conjugated bilirubins are reviewed and recent developments are evaluated. Finally, the present capabilities and future potential of the methods for producing information applicable to the development of new or improved methods of determination are summarized.
准确测定血清中胆红素的种类和含量对于诊断目的以及治疗监测都很重要。然而，在临床实验室常规进行的检测中，胆红素检测并非最准确的，且存在显著的方法差异。在这篇综述中，将讨论血清胆红素的结构、稳定性、溶解性和白蛋白结合特性对分析方法的影响。在考虑分析标准之后，对非结合胆红素和结合胆红素的测定方法进行综述并评估近期的进展。最后，总结了现有方法生成适用于开发新的或改进测定方法的信息的能力以及未来潜力。
