回旋酶突变体中的DNA超螺旋
DNA supercoiling in gyrase mutants.
作者信息
Steck T R, Pruss G J, Manes S H, Burg L, Drlica K
出版信息
J Bacteriol. 1984 May;158(2):397-403. doi: 10.1128/jb.158.2.397-403.1984.
Abstract
Nucleoids isolated from Escherichia coli strains carrying temperature-sensitive gyrA or gyrB mutations were examined by sedimentation in ethidium bromide-containing sucrose density gradients. A shift to restrictive temperature resulted in nucleoid DNA relaxation in all of the mutant strains. Three of these mutants exhibited reversible nucleoid relaxation: when cultures incubated at restrictive temperature were cooled to 0 degree C over a 4- to 5-min period, supercoiling returned to levels observed with cells grown at permissive temperature. Incubation of these three mutants at restrictive temperature also caused nucleoid sedimentation rates to increase by about 50%.
摘要
从携带温度敏感型gyrA或gyrB突变的大肠杆菌菌株中分离出的类核，通过在含溴化乙锭的蔗糖密度梯度中进行沉降来检测。转移至限制温度导致所有突变菌株中的类核DNA松弛。其中三个突变体表现出可逆的类核松弛：当在限制温度下培养的培养物在4至5分钟内冷却至0摄氏度时，超螺旋恢复到在允许温度下生长的细胞所观察到的水平。将这三个突变体在限制温度下孵育也会导致类核沉降速率增加约50%。
相似文献
1
DNA supercoiling in gyrase mutants.
J Bacteriol. 1984 May;158(2):397-403. doi: 10.1128/jb.158.2.397-403.1984.
2
Effects of DNA gyrase inhibitors in Escherichia coli topoisomerase I mutants.
J Bacteriol. 1986 Oct;168(1):276-82. doi: 10.1128/jb.168.1.276-282.1986.
3
New Escherichia coli gyrA and gyrB mutations which have a graded effect on DNA supercoiling.
Mol Gen Genet. 1989 Oct;219(1-2):306-12. doi: 10.1007/BF00261192.
4
Discoordinate gene expression of gyrA and gyrB in response to DNA gyrase inhibition in Escherichia coli.
J Basic Microbiol. 1997;37(1):53-69. doi: 10.1002/jobm.3620370109.
5
Bacterial chromosome segregation: evidence for DNA gyrase involvement in decatenation.
Cell. 1984 Apr;36(4):1081-8. doi: 10.1016/0092-8674(84)90058-8.
6
Topoisomerase mutants and physiological conditions control supercoiling and Z-DNA formation in vivo.
J Biol Chem. 1991 Feb 5;266(4):2576-81.
7
Roles of topoisomerases in maintaining steady-state DNA supercoiling in Escherichia coli.
J Biol Chem. 2000 Mar 17;275(11):8103-13. doi: 10.1074/jbc.275.11.8103.
8
Expression in Escherichia coli of Y5 mutant and N-terminal domain-deleted DNA gyrase B proteins affects strongly plasmid maintenance.
Plasmid. 1998;39(1):21-34. doi: 10.1006/plas.1997.1322.
9
Escherichia coli DNA topoisomerase I mutants: increased supercoiling is corrected by mutations near gyrase genes.
Cell. 1982 Nov;31(1):35-42. doi: 10.1016/0092-8674(82)90402-0.
10
DNA supercoiling in a thermotolerant mutant of Escherichia coli.
Mol Gen Genet. 1995 Aug 30;248(4):417-22. doi: 10.1007/BF02191641.
引用本文的文献
1
Consequences of producing DNA gyrase from a synthetic gyrBA operon in Salmonella enterica serovar Typhimurium.
Mol Microbiol. 2021 Jun;115(6):1410-1429. doi: 10.1111/mmi.14689. Epub 2021 Feb 27.
2
DNA topoisomerases: Advances in understanding of cellular roles and multi-protein complexes via structure-function analysis.
Bioessays. 2021 Apr;43(4):e2000286. doi: 10.1002/bies.202000286. Epub 2021 Jan 22.
3
Rates of gyrase supercoiling and transcription elongation control supercoil density in a bacterial chromosome.
PLoS Genet. 2012;8(8):e1002845. doi: 10.1371/journal.pgen.1002845. Epub 2012 Aug 16.
4
Development of a modified gentamicin resistance cassette for genetic manipulation of the oral spirochete Treponema denticola.
Appl Environ Microbiol. 2012 Mar;78(6):2059-62. doi: 10.1128/AEM.07461-11. Epub 2012 Jan 13.
5
A simplified erythromycin resistance cassette for Treponema denticola mutagenesis.
J Microbiol Methods. 2010 Oct;83(1):66-8. doi: 10.1016/j.mimet.2010.07.020. Epub 2010 Aug 4.
6
Internal structure and dynamics of isolated Escherichia coli nucleoids assessed by fluorescence correlation spectroscopy.
Biophys J. 2007 Apr 15;92(8):2875-84. doi: 10.1529/biophysj.106.095729. Epub 2007 Jan 26.
7
Genomic transcriptional response to loss of chromosomal supercoiling in Escherichia coli.
Genome Biol. 2004;5(11):R87. doi: 10.1186/gb-2004-5-11-r87. Epub 2004 Nov 1.
8
DNA gyrase, topoisomerase IV, and the 4-quinolones.
Microbiol Mol Biol Rev. 1997 Sep;61(3):377-92. doi: 10.1128/mmbr.61.3.377-392.1997.
9
Biochemistry of homologous recombination in Escherichia coli.
Microbiol Rev. 1994 Sep;58(3):401-65. doi: 10.1128/mr.58.3.401-465.1994.
10
Comparison of inhibition of Escherichia coli topoisomerase IV by quinolones with DNA gyrase inhibition.
Antimicrob Agents Chemother. 1994 Nov;38(11):2623-7. doi: 10.1128/AAC.38.11.2623.
本文引用的文献
1
Inhibition of RNA synthesis by oxolinic acid is unrelated to average DNA supercoiling.
J Bacteriol. 1983 Jul;155(1):420-3. doi: 10.1128/jb.155.1.420-423.1983.
2
Regulation of the genes for E. coli DNA gyrase: homeostatic control of DNA supercoiling.
Cell. 1983 Aug;34(1):105-13. doi: 10.1016/0092-8674(83)90140-x.
3
Escherichia coli type-1 topoisomerases: identification, mechanism, and role in recombination.
Cold Spring Harb Symp Quant Biol. 1983;47 Pt 2:769-77. doi: 10.1101/sqb.1983.047.01.088.
4
Positively supercoiled plasmid DNA is produced by treatment of Escherichia coli with DNA gyrase inhibitors.
Nucleic Acids Res. 1983 May 25;11(10):2999-3017. doi: 10.1093/nar/11.10.2999.
5
Escherichia coli DNA topoisomerase I mutants: increased supercoiling is corrected by mutations near gyrase genes.
Cell. 1982 Nov;31(1):35-42. doi: 10.1016/0092-8674(82)90402-0.
6
DNA replication and transcription in a temperature-sensitive mutant of E. coli with a defective DNA gyrase B subunit.
Mol Gen Genet. 1982;188(1):91-5. doi: 10.1007/BF00332999.
7
DNA gyrase is a host factor required for transposition of Tn5.
Cell. 1982 Aug;30(1):9-18. doi: 10.1016/0092-8674(82)90006-x.
8
Essential role of the gyrB gene product in the transcriptional event coupled to dnaA-dependent initiation of Escherichia coli chromosome replication.
Mol Gen Genet. 1981;183(1):134-8. doi: 10.1007/BF00270151.
9
DNA topoisomerases.
Annu Rev Biochem. 1981;50:879-910. doi: 10.1146/annurev.bi.50.070181.004311.
10
Mutations in the gene coding for Escherichia coli DNA topoisomerase I affect transcription and transposition.
Proc Natl Acad Sci U S A. 1981 May;78(5):2747-51. doi: 10.1073/pnas.78.5.2747.
Zotero 插件