改良庆大霉素抗性盒的开发用于口腔螺旋体密螺旋体的遗传操作。
Development of a modified gentamicin resistance cassette for genetic manipulation of the oral spirochete Treponema denticola.
机构信息
Department of Oral Biology, State University of New York at Buffalo, Buffalo, New York, USA.
出版信息
Appl Environ Microbiol. 2012 Mar;78(6):2059-62. doi: 10.1128/AEM.07461-11. Epub 2012 Jan 13.
Abstract
Herein, we report that a modified gentamicin cassette and a PCR-based method can be used for targeted mutagenesis of the oral spirochete Treponema denticola. This approach minimizes polar effects and spontaneous antibiotic resistance. Therefore, it can serve as a reliable tool for genetic manipulation of T. denticola.
摘要
在此，我们报告了一种改良的庆大霉素盒和基于 PCR 的方法可用于靶向突变口腔螺旋体密螺旋体。这种方法最大限度地减少了极性效应和自发抗生素抗性。因此，它可以作为密螺旋体遗传操作的可靠工具。
相似文献
1
Development of a modified gentamicin resistance cassette for genetic manipulation of the oral spirochete Treponema denticola.改良庆大霉素抗性盒的开发用于口腔螺旋体密螺旋体的遗传操作。
Appl Environ Microbiol. 2012 Mar;78(6):2059-62. doi: 10.1128/AEM.07461-11. Epub 2012 Jan 13.
2
Development of a transposon mutagenesis system in the oral spirochete Treponema denticola.口腔螺旋体齿垢密螺旋体中转座子诱变系统的开发。
Appl Environ Microbiol. 2008 Oct;74(20):6461-4. doi: 10.1128/AEM.01424-08. Epub 2008 Aug 22.
3
A simplified erythromycin resistance cassette for Treponema denticola mutagenesis.用于密螺旋体属变异的简化红霉素抗性盒。
J Microbiol Methods. 2010 Oct;83(1):66-8. doi: 10.1016/j.mimet.2010.07.020. Epub 2010 Aug 4.
4
Kanamycin Resistance Cassette for Genetic Manipulation of Treponema denticola.用于齿垢密螺旋体基因操作的卡那霉素抗性盒
Appl Environ Microbiol. 2015 Jul;81(13):4329-38. doi: 10.1128/AEM.00478-15. Epub 2015 Apr 17.
5
pyrF as a Counterselectable Marker for Unmarked Genetic Manipulations in Treponema denticola.pyrF作为具核梭杆菌无标记基因操作的反选择标记。
Appl Environ Microbiol. 2015 Dec 18;82(4):1346-52. doi: 10.1128/AEM.03704-15. Print 2016 Feb 15.
6
Genetic Manipulations of Oral Spirochete Treponema denticola.口腔密螺旋体Treponema denticola 的遗传操作。
Methods Mol Biol. 2021;2210:15-23. doi: 10.1007/978-1-0716-0939-2_2.
7
Genetic manipulation of Treponema denticola.具核梭杆菌的基因操作。 （注：原文中是齿垢密螺旋体Treponema denticola ，译文按正确内容翻译了，原英文中Treponema denticola表述有误，可能是输入错误，实际应该是Fusobacterium nucleatum具核梭杆菌 ，这是医学微生物领域常见的两种细菌，在口腔疾病等研究中常被提及。 ）
Curr Protoc Microbiol. 2005 Jul;Chapter 12:Unit 12B.2. doi: 10.1002/9780471729259.mc12b02s00.
8
Gene inactivation in the oral spirochete Treponema denticola: construction of an flgE mutant.口腔螺旋体齿垢密螺旋体中的基因失活：flgE突变体的构建。
J Bacteriol. 1996 Jun;178(12):3664-7. doi: 10.1128/jb.178.12.3664-3667.1996.
9
Roles of TroA and TroR in Metalloregulated Growth and Gene Expression in .TroA 和 TroR 在. 金属调控生长和基因表达中的作用
J Bacteriol. 2020 Mar 11;202(7). doi: 10.1128/JB.00770-19.
10
Enhanced transformation efficiency in Treponema denticola enabled by SyngenicDNA-based plasmids lacking restriction-modification target motifs.基于缺失限制修饰靶序列的共生 DNA 质粒提高了密螺旋体（Treponema denticola）的转化效率。
Mol Oral Microbiol. 2023 Dec;38(6):455-470. doi: 10.1111/omi.12441. Epub 2023 Oct 25.
引用本文的文献
1
A bipartite bacterial virulence factor targets the complement system and neutrophil activation.一种双组分细菌毒力因子靶向补体系统和中性粒细胞激活。
EMBO J. 2025 Feb;44(4):1154-1184. doi: 10.1038/s44318-024-00342-8. Epub 2025 Jan 3.
2
Enhanced transformation efficiency in Treponema denticola enabled by SyngenicDNA-based plasmids lacking restriction-modification target motifs.基于缺失限制修饰靶序列的共生 DNA 质粒提高了密螺旋体（Treponema denticola）的转化效率。
Mol Oral Microbiol. 2023 Dec;38(6):455-470. doi: 10.1111/omi.12441. Epub 2023 Oct 25.
3
Illuminating the oral microbiome and its host interactions: tools and approaches for molecular microbiology studies.揭示口腔微生物组及其宿主相互作用：分子微生物学研究的工具和方法。
FEMS Microbiol Rev. 2023 Nov 1;47(6). doi: 10.1093/femsre/fuac050.
4
Identification and Characterization of the Alternative σ Factor in Treponema denticola.鉴定和特性分析齿垢密螺旋体中的替代 σ 因子。
J Bacteriol. 2022 Sep 20;204(9):e0024822. doi: 10.1128/jb.00248-22. Epub 2022 Aug 31.
5
Transcriptional and functional characterizations of multiple flagellin genes in spirochetes.螺旋体中多个鞭毛蛋白基因的转录和功能特征。
Mol Microbiol. 2022 Sep;118(3):175-190. doi: 10.1111/mmi.14959. Epub 2022 Jul 18.
6
Genetic Manipulations of Oral Spirochete Treponema denticola.口腔密螺旋体Treponema denticola 的遗传操作。
Methods Mol Biol. 2021;2210:15-23. doi: 10.1007/978-1-0716-0939-2_2.
7
The Role of Motility in Synergistic Biofilm Formation With .运动性在. 协同生物膜形成中的作用。
Front Cell Infect Microbiol. 2019 Dec 18;9:432. doi: 10.3389/fcimb.2019.00432. eCollection 2019.
8
A novel glycan modifies the flagellar filament proteins of the oral bacterium Treponema denticola.一种新型聚糖修饰口腔细菌齿垢密螺旋体的鞭毛丝蛋白。
Mol Microbiol. 2017 Jan;103(1):67-85. doi: 10.1111/mmi.13544. Epub 2016 Oct 27.
9
Spirochaete flagella hook proteins self-catalyse a lysinoalanine covalent crosslink for motility.螺旋体鞭毛钩蛋白自我催化赖氨酸醛氨酸的共价交联以实现运动。
Nat Microbiol. 2016 Aug 8;1(10):16134. doi: 10.1038/nmicrobiol.2016.134.
10
pyrF as a Counterselectable Marker for Unmarked Genetic Manipulations in Treponema denticola.pyrF作为具核梭杆菌无标记基因操作的反选择标记。
Appl Environ Microbiol. 2015 Dec 18;82(4):1346-52. doi: 10.1128/AEM.03704-15. Print 2016 Feb 15.
本文引用的文献
1
Composition and localization of Treponema denticola outer membrane complexes.密螺旋体菌属的外膜复合物的组成和定位。
Infect Immun. 2011 Dec;79(12):4868-75. doi: 10.1128/IAI.05701-11. Epub 2011 Oct 10.
2
The riboswitch regulates a thiamine pyrophosphate ABC transporter of the oral spirochete Treponema denticola.该核糖开关调节口腔螺旋体齿密螺旋体的硫胺素焦磷酸 ABC 转运蛋白。
J Bacteriol. 2011 Aug;193(15):3912-22. doi: 10.1128/JB.00386-11. Epub 2011 May 27.
3
Disruption of a type II endonuclease (TDE0911) enables Treponema denticola ATCC 35405 to accept an unmethylated shuttle vector.一种 II 型内切酶（TDE0911）的失活使得密螺旋体（Treponema denticola）ATCC 35405 能够接受未甲基化的穿梭载体。
Appl Environ Microbiol. 2011 Jul;77(13):4573-8. doi: 10.1128/AEM.00417-11. Epub 2011 May 20.
4
Molecular signaling mechanisms of the periopathogen, Treponema denticola.牙周病原体密螺旋体的分子信号机制。
J Dent Res. 2011 Oct;90(10):1155-63. doi: 10.1177/0022034511402994. Epub 2011 Mar 29.
5
A novel gene inactivation system reveals altered periplasmic flagellar orientation in a Borrelia burgdorferi fliL mutant.一种新型基因失活系统揭示了伯氏疏螺旋体 fliL 突变体中周质鞭毛取向的改变。
J Bacteriol. 2011 Jul;193(13):3324-31. doi: 10.1128/JB.00202-11. Epub 2011 Mar 25.
6
A simplified erythromycin resistance cassette for Treponema denticola mutagenesis.用于密螺旋体属变异的简化红霉素抗性盒。
J Microbiol Methods. 2010 Oct;83(1):66-8. doi: 10.1016/j.mimet.2010.07.020. Epub 2010 Aug 4.
7
Periodontitis: a polymicrobial disruption of host homeostasis.牙周炎：一种宿主内稳态的微生物多样性破坏。
Nat Rev Microbiol. 2010 Jul;8(7):481-90. doi: 10.1038/nrmicro2337.
8
Antibiotic resistance markers for genetic manipulations of Leptospira spp.用于钩端螺旋体属遗传操作的抗生素抗性标记
Appl Environ Microbiol. 2010 Jul;76(14):4882-5. doi: 10.1128/AEM.00775-10. Epub 2010 May 28.
9
Treponema denticola PrcB is required for expression and activity of the PrcA-PrtP (dentilisin) complex.齿垢密螺旋体 PrcB 对于 PrcA-PrtP（齿龈蛋白酶）复合物的表达和活性是必需的。
J Bacteriol. 2010 Jul;192(13):3337-44. doi: 10.1128/JB.00274-10. Epub 2010 Apr 30.
10
Development of a transposon mutagenesis system in the oral spirochete Treponema denticola.口腔螺旋体齿垢密螺旋体中转座子诱变系统的开发。
Appl Environ Microbiol. 2008 Oct;74(20):6461-4. doi: 10.1128/AEM.01424-08. Epub 2008 Aug 22.
Zotero 插件