Department of Oral Biology, State University of New York at Buffalo, Buffalo, New York, USA.
Appl Environ Microbiol. 2012 Mar;78(6):2059-62. doi: 10.1128/AEM.07461-11. Epub 2012 Jan 13.
Herein, we report that a modified gentamicin cassette and a PCR-based method can be used for targeted mutagenesis of the oral spirochete Treponema denticola. This approach minimizes polar effects and spontaneous antibiotic resistance. Therefore, it can serve as a reliable tool for genetic manipulation of T. denticola.
在此,我们报告了一种改良的庆大霉素盒和基于 PCR 的方法可用于靶向突变口腔螺旋体密螺旋体。这种方法最大限度地减少了极性效应和自发抗生素抗性。因此,它可以作为密螺旋体遗传操作的可靠工具。