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大肠杆菌K-12中的D-核糖代谢：遗传学、调控与转运

D-ribose metabolism in Escherichia coli K-12: genetics, regulation, and transport.

作者信息

Lopilato J E, Garwin J L, Emr S D, Silhavy T J, Beckwith J R

出版信息

J Bacteriol. 1984 May;158(2):665-73. doi: 10.1128/jb.158.2.665-673.1984.

DOI:10.1128/jb.158.2.665-673.1984
PMID:6327616
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC215481/
Abstract

We have isolated mutants defective in high-affinity D-ribose transport. The mutations map in rbsT or rbsB , the structural gene for ribose binding protein. rbsT consists of at least one gene coding for a protein required for high-affinity transport. The high-affinity transport-defective mutants were able to utilize D-ribose, indicating that at least a second, low-affinity transport system for D-ribose is present in Escherichia coli K-12. rbsT and rbsB are located at min 84 on the E. coli genetic map and, together with rbsK , the gene coding for ribokinase , constitute an rbs operon. The order of genes is rbsP /O rbsT rbsB rbsK . The rbs operon is subject to negative control by the product of the rbsR gene. rbsR is located distal to the rbs operon and appears to form a separate transcriptional unit.

摘要

我们已经分离出了高亲和力D-核糖转运存在缺陷的突变体。这些突变定位于rbsT或rbsB，即核糖结合蛋白的结构基因。rbsT至少由一个编码高亲和力转运所需蛋白质的基因组成。高亲和力转运缺陷型突变体能够利用D-核糖，这表明大肠杆菌K-12中至少存在第二个低亲和力D-核糖转运系统。rbsT和rbsB位于大肠杆菌遗传图谱的84分钟处，并且与编码核糖激酶的基因rbsK一起构成一个rbs操纵子。基因顺序为rbsP/O rbsT rbsB rbsK。rbs操纵子受到rbsR基因产物的负调控。rbsR位于rbs操纵子的远端，似乎形成一个单独的转录单元。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccb5/215481/87032c9c7e53/jbacter00234-0283-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccb5/215481/cbe124fcbedd/jbacter00234-0279-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccb5/215481/8c699b490905/jbacter00234-0280-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccb5/215481/16f9241915cf/jbacter00234-0283-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccb5/215481/87032c9c7e53/jbacter00234-0283-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccb5/215481/cbe124fcbedd/jbacter00234-0279-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccb5/215481/8c699b490905/jbacter00234-0280-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccb5/215481/16f9241915cf/jbacter00234-0283-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ccb5/215481/87032c9c7e53/jbacter00234-0283-b.jpg

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