Busse W W, Kopp D E, Middleton E
J Allergy Clin Immunol. 1984 Jun;73(6):801-9. doi: 10.1016/0091-6749(84)90450-0.
Flavonoids are naturally occurring plant compounds that have been demonstrated to possess a variety of anti-inflammatory effects. We studied the effects of flavonoids on three aspects of neutrophil function that are commonly considered to be associated with inflammation: the release of lysosomal enzymes, the chemiluminescence (CL) response, and the production of superoxide anion. Quercetin and eight other flavonoids at a 10(-5)M concentration inhibited the neutrophil CL response to opsonized zymosan particles by approximately 60% or more. In contrast, the release of lysosomal beta-glucuronidase from neutrophils stimulated with opsonized zymosan was only inhibited by two flavonoids, quercetin and chalcone, and only at concentrations of 1.5 X 10(-4)M to 2 X 10(-4)M. Quercetin also inhibited the generation of superoxide anion by neutrophils but to a lesser degree than its effect on CL. The present studies demonstrated that certain flavonoids are not uniformly active in inhibiting neutrophil CL, beta-glucuronidase release, or superoxide generation. The effects of flavonoids on neutrophil functions probably depend on many variables including the response measured, the activating stimulus, and specific flavonoid structural features.
黄酮类化合物是天然存在的植物化合物，已被证明具有多种抗炎作用。我们研究了黄酮类化合物对中性粒细胞功能三个方面的影响，这三个方面通常被认为与炎症相关：溶酶体酶的释放、化学发光（CL）反应以及超氧阴离子的产生。槲皮素和其他八种浓度为10^(-5)M的黄酮类化合物可使中性粒细胞对调理酵母聚糖颗粒的CL反应抑制约60%或更多。相比之下，经调理酵母聚糖刺激的中性粒细胞溶酶体β-葡萄糖醛酸酶的释放仅受到两种黄酮类化合物（槲皮素和查耳酮）的抑制，且仅在浓度为1.5×10^(-4)M至2×10^(-4)M时才会出现这种抑制作用。槲皮素也抑制中性粒细胞产生超氧阴离子，但其抑制程度小于对CL的影响。目前的研究表明，某些黄酮类化合物在抑制中性粒细胞CL、β-葡萄糖醛酸酶释放或超氧阴离子生成方面并非均一有效。黄酮类化合物对中性粒细胞功能的影响可能取决于许多变量，包括所测量的反应、激活刺激以及特定的黄酮类化合物结构特征。
