Shibayama T, Watanabe T, Kojima H, Yoshikawa A, Watanabe S, Kamimura T, Suzuki S, Ichida F
J Med Virol. 1984;13(3):205-14. doi: 10.1002/jmv.1890130302.
Electron microscopic studies of the morphology of hepatitis B surface antigen (HBsAg) produced by PLC/PRF/5 cells in vitro were carried out. Aggregates of 20-nm spherical particles in 3-day culture supernatants were observed by immune electron microscopy (IEM). Aggregates of tubular structures were found with IEM in the extracts of the cells. Tubular structures 18 to 22 nm in diameter were seen by electron microscopy (EM) in the cisternae of the endoplasmic reticulum in 2-3% of the cells. The tubular structures in the cytoplasm and extracts of PLC/PRF/5 cells resembled those observed in the hepatocytes of human carriers of hepatitis B virus (HBV). Intracellular localization of HBsAg in PLC/PRF/5 cells by direct peroxidase-conjugated antibody staining was observed on the tubular structures and the cisternal wall, which contained these structures. Rotation technique analysis indicated that the tubular structures were composed of 11 or 12 subunits.
对PLC/PRF/5细胞体外产生的乙型肝炎表面抗原（HBsAg）形态进行了电子显微镜研究。通过免疫电子显微镜（IEM）观察到3天培养上清液中有20纳米球形颗粒的聚集体。在细胞提取物中用IEM发现了管状结构的聚集体。在2%-3%的细胞内质网池内，通过电子显微镜（EM）观察到直径为18至22纳米的管状结构。PLC/PRF/5细胞的细胞质和提取物中的管状结构与在乙型肝炎病毒（HBV）携带者的肝细胞中观察到的相似。通过直接过氧化物酶偶联抗体染色观察到PLC/PRF/5细胞中HBsAg的细胞内定位在管状结构和含有这些结构的池壁上。旋转技术分析表明管状结构由11或12个亚基组成。
