Almeida J D, Bradburne A F
J Med Virol. 1984;13(3):301-12. doi: 10.1002/jmv.1890130312.
Immune electron microscopy has been used to examine the appearance of three-component complexes. The three components are antigen, supplied by two dissimilar viruses, antibody, and a secondary immune reactant. Secondary reagents used in the study are antispecies immunoglobulin (anti-IgG), rheumatoid factor (RF), and complement. Each of these secondary reagents produced cross-linking between antigenically unrelated immune complexes, and it was found possible to distinguish visually the mixed complexes produced by each of them. The significance of the appearance of these mixed complexes is discussed and related to the neutralisation enhancement that can occur in the presence of secondary immune reactants. The appearance of the complexes is also related to the false positive results that can be obtained in carrying out solid phase immunoassays.
免疫电子显微镜已被用于检测三元复合物的外观。这三种成分分别是由两种不同病毒提供的抗原、抗体和二级免疫反应物。该研究中使用的二级试剂是抗种属免疫球蛋白（抗IgG）、类风湿因子（RF）和补体。这些二级试剂中的每一种都能在抗原无关的免疫复合物之间产生交联，并且发现可以通过视觉区分由它们各自产生的混合复合物。文中讨论了这些混合复合物外观的意义，并将其与在二级免疫反应物存在下可能发生的中和增强联系起来。复合物的外观还与进行固相免疫测定时可能获得的假阳性结果有关。
