L'Abbé M R, Fischer P W
J Nutr. 1984 May;114(5):813-22. doi: 10.1093/jn/114.5.813.
The effects of feeding rats high levels of dietary zinc (240 mg Zn/kg diet) on the activities of the copper-requiring metalloenzymes: ceruloplasmin (Cp), cupro -zinc superoxide dismutase (SOD) and cytochrome c oxidase (CCO) were determined. These were compared with those seen during copper deficiency (induced by feeding 0.6 mg Cu/kg diet). The activities of Cp in serum, Cu-Zn SOD in liver and heart, and CCO in heart were significantly reduced in both the high zinc and copper-deficient groups by 2 weeks, when compared to the activities seen in rats fed the control diet (6 mg Cu, 30 mg Zn/kg diet). In animals fed the high zinc diet, the reduction in heart CCO activity followed the decrease seen in heart copper concentration, whereas in blood and liver, the reductions in Cp and SOD activities, respectively, were greater than the reductions seen in copper concentration. Thus, the results of this experiment demonstrate that with high dietary zinc, the reductions seen in the activity of copper-requiring metalloenzymes were similar to those seen during copper deficiency.
测定了给大鼠喂食高水平膳食锌（240毫克锌/千克日粮）对需要铜的金属酶（即铜蓝蛋白（Cp）、铜锌超氧化物歧化酶（SOD）和细胞色素c氧化酶（CCO））活性的影响。将这些结果与铜缺乏（通过喂食0.6毫克铜/千克日粮诱导）期间观察到的结果进行了比较。与喂食对照日粮（6毫克铜、30毫克锌/千克日粮）的大鼠相比，高锌组和缺铜组血清中的Cp、肝脏和心脏中的铜锌SOD以及心脏中的CCO活性在2周时均显著降低。在喂食高锌日粮的动物中，心脏CCO活性的降低与心脏铜浓度的降低一致，而在血液和肝脏中，Cp和SOD活性的降低分别大于铜浓度的降低。因此，本实验结果表明，高膳食锌时，需要铜的金属酶活性的降低与铜缺乏时观察到的情况相似。
