Paynter D I, Moir R J, Underwood E J
J Nutr. 1979 Sep;109(9):1570-6. doi: 10.1093/jn/109.9.1570.
The effects of dietary copper level on tissue activities of the copper containing superoxide dismutase (CuSOD) were investigated, and these activities related to those of other copper containing enzymes particularly cytochrome oxidase. Male weaning rats were fed a basal diet (containing 0.8 mg Cu/kg) or this diet supplemented with 4 or 24 mg Cu/kg. After 6 weeks, rats fed the basal diet were then repleted using the high copper diet. In the two copper supplemented groups, no differences were observed in any of the parameters measured. In these groups, tissue activities of CuSOD were in the order of liver greater than kidney greater than RBC greater than testis greater than heart greater than brain greater than lung greater than muscle. In the basal group, CuSOD activity decreased in liver; RBC and heart to 14, 25, and 61%, respectively, of control activities after 6 weeks' depletion; tissues other than brain or muscle showed smaller but significant changes. Conversely, heart and muscle cytochrome oxidase activities decreased to 30 and 45% of control activity and liver to 70%. With repletion, CuSOD activities in liver and heart increased more rapidly than did cytochrome oxidase activities. It is concluded that liver CuSOD activity, which is normally high, is greatly reduced with little change in cytochrome oxidase activity; the reverse is found for heart and muscle tissue. The relevance of these changes to the maintenance of tissue integrity is discussed.
研究了日粮铜水平对含铜超氧化物歧化酶（CuSOD）组织活性的影响，以及这些活性与其他含铜酶特别是细胞色素氧化酶活性的关系。给雄性断奶大鼠喂食基础日粮（含铜0.8毫克/千克）或添加了4或24毫克/千克铜的该日粮。6周后，给喂食基础日粮的大鼠改用高铜日粮。在两个添加铜的组中，所测的任何参数均未观察到差异。在这些组中，CuSOD的组织活性顺序为肝脏＞肾脏＞红细胞＞睾丸＞心脏＞大脑＞肺＞肌肉。在基础组中，肝脏中的CuSOD活性降低；红细胞和心脏在6周耗竭后分别降至对照活性的14%、25%和61%；大脑或肌肉以外的组织显示出较小但显著的变化。相反，心脏和肌肉的细胞色素氧化酶活性分别降至对照活性的30%和45%，肝脏降至70%。补充铜后，肝脏和心脏中CuSOD的活性比细胞色素氧化酶活性增加得更快。得出的结论是，正常情况下较高的肝脏CuSOD活性在细胞色素氧化酶活性变化不大的情况下大幅降低；心脏和肌肉组织则相反。讨论了这些变化与维持组织完整性的相关性。
