来自猿猴病毒40转化的人类细胞系猿猴病毒80的突变T抗原的DNA结合特性。
DNA binding properties of a mutant T antigen from the simian virus 40-transformed human cell line simian virus 80.
作者信息
Gruss C, Baumann E, Knippers R
出版信息
J Virol. 1984 Jun;50(3):943-6. doi: 10.1128/JVI.50.3.943-946.1984.
Abstract
We investigated whether the T antigen of the simian virus 40-transformed human cell line simian virus 80 ( SV80 ) specifically recognizes DNA sequences of its own template, i.e, the viral sequences integrated in the SV80 cellular genome. In vitro DNA binding experiments clearly indicated that, in contrast to wild-type T antigen, SV80 T antigen does not specifically bind to sites on the integrated viral DNA in SV80 cells.
摘要
我们研究了猿猴病毒40转化的人类细胞系猿猴病毒80（SV80）的T抗原是否能特异性识别其自身模板的DNA序列，即整合在SV80细胞基因组中的病毒序列。体外DNA结合实验清楚地表明，与野生型T抗原不同，SV80 T抗原不会特异性结合SV80细胞中整合的病毒DNA上的位点。
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