Both G W, Siegman L J, Bellamy A R, Ikegami N, Shatkin A J, Furuichi Y
J Virol. 1984 Jul;51(1):97-101. doi: 10.1128/JVI.51.1.97-101.1984.
Cloned DNA copies of rotavirus genomic segment 6 from simian 11 (subgroup 1) and human strain Wa (subgroup 2) rotaviruses have been used to determine the nucleotide sequences of the gene that determines viral subgroup specificity. Both genomic segments are 1,356 nucleotides in length and possess 5'- and 3'-terminal untranslated regions of 23 and 142 nucleotides, respectively. The inferred amino acid sequence reveals VP6 to be a polypeptide of 397 amino acids in which more than 90% of the amino acid sequence is conserved between the two viruses. There are 34 amino acid changes between the subgroup 1 and 2 polypeptides, most clustered in three regions of the molecule at residues 39 through 62, 80 through 122, and 281 through 315.
来自猿猴11型(第1亚组)和人类轮状病毒Wa株(第2亚组)的轮状病毒基因组片段6的克隆DNA拷贝已被用于确定决定病毒亚组特异性的基因的核苷酸序列。两个基因组片段长度均为1356个核苷酸,分别拥有长度为23个和142个核苷酸的5'和3'末端非翻译区。推断的氨基酸序列显示VP6是一种由397个氨基酸组成的多肽,其中两种病毒之间90%以上的氨基酸序列是保守的。第1亚组和第2亚组多肽之间有34个氨基酸变化,大多数集中在分子的三个区域,即第39至62位、第80至122位和第281至315位残基处。