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葡萄糖会抑制酿酒酵母中编码线粒体成分的核基因的转录。

Glucose represses transcription of Saccharomyces cerevisiae nuclear genes that encode mitochondrial components.

作者信息

Szekely E, Montgomery D L

出版信息

Mol Cell Biol. 1984 May;4(5):939-46. doi: 10.1128/mcb.4.5.939-946.1984.

DOI:10.1128/mcb.4.5.939-946.1984
PMID:6328277
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC368844/
Abstract

By Northern blot hybridization analysis, we demonstrated that the steady-state levels of mRNAs specifying the alpha subunit of ATPase, the beta subunit of ATPase, and the ATP/ADP translocator are all reduced in cells grown in glucose-rich medium. The extent to which glucose represses the levels of alpha, beta, and translocator mRNAs varies from strain to strain, from 2.5- to 7-fold. Furthermore, by hybridization experiments with an excess of DNA, we showed that glucose represses the rates of synthesis of these mRNAs. The kinetics of repression and depression of transcription were also studied. Finally, a mutant was characterized which appears to be defective in depression of transcription of the genes encoding the alpha and beta ATPase subunits as well as the ATP/ADP translocator.

摘要

通过Northern印迹杂交分析，我们证明，在富含葡萄糖的培养基中生长的细胞中，编码ATP酶α亚基、ATP酶β亚基和ATP/ADP转位酶的mRNA的稳态水平均降低。葡萄糖对α、β和转位酶mRNA水平的抑制程度因菌株而异，为2.5至7倍。此外，通过用过量DNA进行杂交实验，我们表明葡萄糖抑制这些mRNA的合成速率。还研究了转录抑制和去抑制的动力学。最后，对一个突变体进行了表征，该突变体在编码α和βATP酶亚基以及ATP/ADP转位酶的基因转录去抑制方面似乎存在缺陷。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/247d/368844/2e4dd9198230/molcellb00147-0134-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/247d/368844/da8651395ae1/molcellb00147-0130-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/247d/368844/4a4ad648dae3/molcellb00147-0131-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/247d/368844/ab14bc0ac294/molcellb00147-0133-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/247d/368844/67456ddb722f/molcellb00147-0133-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/247d/368844/2e4dd9198230/molcellb00147-0134-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/247d/368844/da8651395ae1/molcellb00147-0130-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/247d/368844/4a4ad648dae3/molcellb00147-0131-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/247d/368844/ab14bc0ac294/molcellb00147-0133-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/247d/368844/67456ddb722f/molcellb00147-0133-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/247d/368844/2e4dd9198230/molcellb00147-0134-a.jpg

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本文引用的文献

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