皮肤癣菌分类学中的DNA同源性研究。

DNA homology studies in the taxonomy of dermatophytes.

作者信息

Davison F D, Mackenzie D W

出版信息

Sabouraudia. 1984;22(2):117-23. doi: 10.1080/00362178485380191.

Abstract

The taxonomy of the dermatophyte genera Trichophyton, Microsporum and Epidermophyton was investigated by determinations of DNA homology. DNA extracted from 10 isolates (seven species) of dermatophytes was reannealed by hydroxyapatite chromatography. DNA homology levels of 65-80% corresponded to species within a single genus. There was a general agreement with established classification, particularly in assigning species to separate genera. DNA from the Trichophyton state of Arthroderma benhamiae (NCPF 456) showed 71 and 76% homology with DNA from T. mentagrophytes (NCPF 224) and T. rubrum (NCPF 5034) respectively. T. terrestre showed low homology (25%) when compared with the other species of dermatophytes tested.

摘要

通过测定DNA同源性对皮肤癣菌属毛癣菌属、小孢子菌属和表皮癣菌属进行了分类研究。从10株皮肤癣菌（7个种）分离株中提取的DNA经羟基磷灰石层析进行重退火。65%-80%的DNA同源性水平对应于单个属内的种。与既定分类总体一致，特别是在将种归入不同属方面。来自贝氏节皮菌（NCPF 456）毛癣菌状态的DNA分别与须癣毛癣菌（NCPF 224）和红色毛癣菌（NCPF 5034）的DNA显示出71%和76%的同源性。与所测试的其他皮肤癣菌种相比，土生毛癣菌显示出较低的同源性（25%）。