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本文引用的文献

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Primer Design Assistant (PDA): A web-based primer design tool.引物设计助手(PDA):一个基于网络的引物设计工具。
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2
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J Clin Microbiol. 2002 Dec;40(12):4804-5. doi: 10.1128/JCM.40.12.4804-4805.2002.
3
PCR identification of dermatophyte fungi Trichophyton rubrum, T. soudanense and T. gourvilii.红色毛癣菌、苏丹毛癣菌和古尔维利毛癣菌的聚合酶链反应鉴定
J Med Microbiol. 2002 Feb;51(2):117-122. doi: 10.1099/0022-1317-51-2-117.
4
Tinea capitis in adults during 1981-95 in northern Greece.1981年至1995年希腊北部成年人头癣情况。
Mycoses. 2001 Nov;44(9-10):398-400. doi: 10.1046/j.1439-0507.2001.00675.x.
5
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J Clin Microbiol. 2001 Sep;39(9):3382-5. doi: 10.1128/JCM.39.9.3382-3385.2001.
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A specific PCR assay for the dermatophyte fungus Microsporum canis.犬小孢子菌皮肤癣菌的特异性聚合酶链反应检测方法。
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8
Epidemiology of tinea capitis.头癣的流行病学
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[Epidemiology and ecology of dermatophytoses in the City of Fortaleza: Trichophyton tonsurans as important emerging pathogen of Tinea capitis].[福塔莱萨市皮肤癣菌病的流行病学与生态学:须癣毛癣菌作为头癣的重要新兴病原体]
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Molecular taxonomy of the Trichophyton rubrum complex.红色毛癣菌复合体的分子分类学
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用于在临床样本中通过聚合酶链反应快速检测奥杜盎小孢子菌的特异性引物。

Specific primers for rapid detection of Microsporum audouinii by PCR in clinical samples.

作者信息

Roque H D, Vieira R, Rato S, Luz-Martins M

机构信息

Lab. Micologia, Instituto de Higiene e Medicina Tropical/CREM, Universidade Nova de Lisboa, Portugal.

出版信息

J Clin Microbiol. 2006 Dec;44(12):4336-41. doi: 10.1128/JCM.00759-06. Epub 2006 Sep 27.

DOI:10.1128/JCM.00759-06
PMID:17005755
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1698423/
Abstract

This report describes application of PCR fingerprinting to identify common species of dermatophytes using the microsatellite primers M13, (GACA)4, and (GTG)5. The initial PCR analysis rendered a specific DNA fragment for Microsporum audouinii, which was cloned and sequenced. Based on the sequencing data of this fragment, forward (MA_1F) and reverse (MA_1R) primers were designed and verified by PCR to establish their reliability in the diagnosis of M. audouinii. These primers produced a singular PCR band of 431 bp specific only to strains and isolates of M. audouinii, based on a global test of 182 strains/isolates belonging to 11 species of dermatophytes. These findings indicate these primers are reliable for diagnostic purposes, and we recommend their use in laboratory analysis.

摘要

本报告描述了利用微卫星引物M13、(GACA)4和(GTG)5,通过聚合酶链反应(PCR)指纹图谱技术来鉴定皮肤癣菌常见种类的应用。最初的PCR分析产生了一个针对奥杜盎小孢子菌的特定DNA片段,该片段被克隆并测序。基于该片段的测序数据,设计了正向引物(MA_1F)和反向引物(MA_1R),并通过PCR验证以确定它们在诊断奥杜盎小孢子菌中的可靠性。基于对属于11种皮肤癣菌的182个菌株/分离株的全面测试,这些引物产生了一条仅对奥杜盎小孢子菌的菌株和分离株特异的431 bp单一PCR条带。这些结果表明这些引物用于诊断目的是可靠的,我们建议在实验室分析中使用它们。