Tunkara A, Ignatova M, Vinarova M, Runevski N
Vet Med Nauki. 1984;21(2):40-8.
Parallel cytomorphologic and cytochemical studies were carried out on the replication of the bovine reovirus type 1 in the Vero cell line. It was found that a high multiplicity of infection M = 5 the first morphologic changes took place some 12 hours following infection, while at the 24th hour there appeared also the characteristic inclusion bodies. At the same time the appearance was noted of acidophile masses in the cytoplasm of some of the infected cells. The morphologic changes reached the peak values of their development at the 48th hour, and the cytoplasmic inclusions assumed a net-shaped structure of lower density. It was also found that following a short-term rise in the first 12 hours after infection there set in a general suppression of the RNA-containing material, which spread irregularly through-out the cell thus marking the nonpyroninophile viral inclusions. The desoxiribonucleic synthesis was not visibly affected, however, the nuclear chromatin remained with an unchanged structure. The viral inclusions were Feulgen PAS-negative. The hydrolytic enzymes alkaline and acid phosphatase and 5-nucleotidase were strongly inhibited after a short-term rise within the first 12 hours of infection.
对1型牛呼肠孤病毒在Vero细胞系中的复制进行了平行的细胞形态学和细胞化学研究。结果发现，在高感染复数M = 5时，感染后约12小时出现首次形态学变化，而在第24小时也出现了特征性包涵体。同时，在一些感染细胞的细胞质中注意到嗜酸性团块的出现。形态学变化在第48小时达到其发展的峰值，细胞质包涵体呈现出低密度的网状结构。还发现，在感染后的前12小时内短期上升之后，含RNA物质普遍受到抑制，其在整个细胞中不规则分布，从而标记出非嗜派洛宁病毒包涵体。然而，脱氧核糖核酸合成未受到明显影响，核染色质结构保持不变。病毒包涵体对福尔根PAS呈阴性。在感染后的前12小时内短期上升之后，水解酶碱性磷酸酶、酸性磷酸酶和5-核苷酸酶受到强烈抑制。
