Metzler A E, Krakowka S, Axthelm M K, Gorham J R
Am J Vet Res. 1984 Oct;45(10):2211-5.
Primary cultures of bovine fibroblast (BF) and canine brain cells, persistently infected with virulent R252-canine distemper virus (CDV), were cocultured with African green monkey (Vero) cells. Transfer of persistent CDV from BF to Vero cells varied inversely with the in vitro passage level (age) of the CDV-infected BF cells. Successful transfer of CDV to Vero cells was signaled by the transient appearance of viral syncytia, rapid spread of viral antigen to all Vero cells in the culture, and by recovery of cell-free Vero-infectious virus in culture fluids. With time, viral cytopathic effects in Vero cells containing CDV disappeared, and the infected lines could not be distinguished from noninfected control Vero cells, except by immunoassay for viral antigen.
用强毒株R252 - 犬瘟热病毒（CDV）持续感染的牛成纤维细胞（BF）和犬脑细胞的原代培养物，与非洲绿猴（Vero）细胞进行共培养。持续性CDV从BF细胞向Vero细胞的转移与CDV感染的BF细胞的体外传代水平（代数）呈反比。CDV成功转移至Vero细胞的标志是病毒多核巨细胞的短暂出现、病毒抗原迅速扩散至培养物中的所有Vero细胞，以及在培养液中回收无细胞的可感染Vero细胞的病毒。随着时间推移，含有CDV的Vero细胞中的病毒细胞病变效应消失，除通过病毒抗原免疫测定外，感染的细胞系无法与未感染的对照Vero细胞区分开来。
