Vainstein A, Hershkovitz M, Israel S, Rabin S, Loyter A
Biochim Biophys Acta. 1984 Jun 27;773(2):181-8. doi: 10.1016/0005-2736(84)90081-6.
A new way for reconstituting highly fusogenic Sendai virus envelopes is described. As opposed to previously described methods, in the present one the detergent (Triton X-100) is removed by direct addition of SM-2 Bio-beads to the detergent solubilized mixture of the viral phospholipids and glycoproteins, thus avoiding the long dialysis step. The vesicles obtained in the present work resemble, in their composition, size and features, envelopes of intact Sendai virus particles. The present method allows the enclosure of low and high molecular weight material within the reconstituted viral envelopes.
描述了一种重组高融合性仙台病毒包膜的新方法。与先前描述的方法不同，在本方法中，通过直接向病毒磷脂和糖蛋白的去污剂溶解混合物中添加SM-2生物珠来去除去污剂（Triton X-100），从而避免了漫长的透析步骤。在本研究中获得的囊泡在其组成、大小和特征方面类似于完整仙台病毒颗粒的包膜。本方法允许将低分子量和高分子量物质包裹在重组病毒包膜内。
